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Every laboratory worker who takes an occasional tion has experienced the vexation of having no hematoxylin in perfect working order on his return home. Bythe usual methods of preparing this stain one is compelled to wait several days for the stain to ripen. To eliminate this the following formula may be successfully used.
Hematoxylin crystals..... 1 gm.A Alcohol.................. 10 c. c. Alum................... 10 gm. B Distilled water........... 200 c.c. Hydrogen peroxid, q. s.
The hematoxylin crystals are thoroughly pulverized in a mortar and the alcohol added; the alum is dissolved in the distilled water. A is then added slowly with constant stirring to B. When well mixed, hydrogen peroxid is added drop by drop till the color changes to a deep purple. After filtering, the stain is ready for immediate use. The only advantage of this method is the rapidity of preparation. The entire ".""j|)m,( se*'* in preparing a stain
JOHNSTON JA. RAPID PREPARATION OF HEMATOXYLIN STAIN. JAMA. 1911;LVII(25):1988. doi:10.1001/jama.1911.04260120178015