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Article
May 9, 1914

A METHOD OF STAINING AND MOUNTING SMEARS

Author Affiliations

Minneapolis

JAMA. 1914;LXII(19):1473. doi:10.1001/jama.1914.02560440029010

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Abstract

Time, expense and simplicity are large factors in laboratory work, especially when the practitioner does the work himself. In the preparation of smears of gastric extract and feces 1 have been using, for some months, a method which not only fulfils the foregoing requirements, but also can be adapted to the staining and mounting of smears from many other sources. The idea was suggested to me by Smithies' agar preparation, the disadvantages of which are that agar is very slow to put into solution and must be kept in a refrigerator to preserve for any length of time. I have substituted ordinary powdered gelatin, which goes into immediate solution in boiling water. A few pinches of gelatin powder are added to a small quantity of boiling water in a test-tube. For immediate use 2 or 3 c.c. are poured into a smaller tube and about one-third the volume of stain

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