Nicolaus Afanassieff1 of Moscow undertook to study the reactive changes that occur in granulation tissue when pathogenic bacteria are introduced into it. Some of the results obtained seem of more than ordinary interest and importance because they touch one of the most interesting questions in biology, namely, the nature of the struggle, if one may use the expression, of the organism against infection.
While very many investigators have studied the ability of granulation tissue to absorb alkaloids, various other chemic substances, and infusions of decomposing materials, none of the previous researches have taken up the question, under what conditions does infection with pathogenic bacteria of the whole organism take place through granulation tissue. Afanassieff made it his object to study the consequences of experimental infection of granulation tissue with various pathogenic microbes. After trying several methods it was found, by excising a small piece of skin with the subcutaneous
THE ROLE OF GRANULATION TISSUE IN WOUND INFECTION WITH PATHOGENIC BACTERIA.. JAMA. 1897;XXIX(15):756–757. doi:10.1001/jama.1897.02440410044007