In a former paper1 I pointed out that a solution of methylene violet (Bernthsen's), methylene blue and eosin in methyl alcohol makes an excellent stain for blood films, and suggested the use of such a solution for staining Spirochæta pallida. With the very limited number of film preparations then at hand, some fair results were obtained by this method. Recently it has been possible to get a large number of films from a case of primary syphilis and to test more thoroughly this stain. The result has been so good that it seems the method may be of value in diagnosis.
The case occurred in the practice of Dr. Simpson, and he has very kindly furnished the following history:
—Male, aged 21, presented himself Jan. 2, 1902, on account of a sore on penis.
—Father living and well; mother dead of tuberculosis; one brother and one sister
MACNEAL WJ. A RAPID AND SIMPLE METHOD OF STAINING SPIROCHÆTA PALLIDA.. JAMA. 1907;XLVIII(7):609-610. doi:10.1001/jama.1907.25220330051002b