[Skip to Content]
[Skip to Content Landing]
Article
April 13, 1907

A CONVENIENT METHOD OF OBTAINING CLUMP-FREE EMULSION FOR OPSONIC WORK.

Author Affiliations

MONROVIA, CAL.

JAMA. 1907;XLVIII(15):1269. doi:10.1001/jama.1907.25220410045002g

This article is only available in the PDF format. Download the PDF to view the article, as well as its associated figures and tables.

Abstract

While studying the specificity of opsonins last summer in Professor Wright's laboratory I sought for a method of quickly separating organisms from the liquid in which they are suspended, centrifugating alone requiring from thirty minutes to one hour. The following device was used with splendid success: A constriction (A) is made in a piece of small-caliber glass tubing; the tubing is then bent on itself (B), terminating in an opening (C). Moist filter paper fiber (D) (conveniently made by scraping filter paper with the edge of a knife) is placed against the constriction and packed firmly. Into the long arm is placed the liquid (E) to be filtered and the device is placed in the centrifuge.

Working with staphylococci, I obtained practically bacteria-free filtrates (F) in ten to fifteen minutes from thick suspensions of staphylococci in normal sera. The filtrates showed scarcely any opsonin, and stained specimens practically no organisms.

First Page Preview View Large
First page PDF preview
First page PDF preview
×