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The rapid increase in the microscope's application to scientific study has necessitated that every microscopist collect specimens of all the interesting objects with which he may meet in laboratory work. Furthermore, it is far more satisfactory to preserve the original specimens as a referencce than to depend on notes, which, aside from the time employed in writing and reading, are always unsatisfactory. In the teaching of chemistry, botany, histology, pathology, bacteriology, and clinical microscopy it is necessary that the student prepare a specimen from each subject studied, both as a reference specimen and to demonstrate, beyond a doubt, that he has mastered this art. In board of health laboratories it is necessary to preserve specimen slides as an assurance against criticism, and for this reason most of the bacteriological work is now done by fixing and staining the specimen on the slides.
The leading objection to the staining of specimens
BOSTON LN. Forceps for Holding Slides While Preparing Microscopic Specimens.. JAMA. 1900;XXXV(10):641. doi:10.1001/jama.1900.24620360033021a