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Article
March 22, 1965

Isolation and Storage of Artificially Oxygenated Mammalian Hearts

Author Affiliations

From the Department of Surgery, Washington University School of Medicine and Barnes Hospital, St. Louis.

JAMA. 1965;191(12):1006-1008. doi:10.1001/jama.1965.03080120040010
Abstract

A satisfactory method by which viable and uninjured mammalian hearts may be removed and stored is important to the study of cardiac homotransplantation. This report describes a method of cardiac isolation and storage.

Methods  All animals were anesthetized with pentobarbital (30 mg/kg) and ventilated by an automatic respirator using room air. Series 1 consisted of 24 experiments using 48 mongrel dogs (10 to 20 kg) to study the technical aspects of cardiac isolation, storage, and parallel transplantation. Series 2 consisted of 16 experiments using 16 mongrel dogs (10 to 23 kg) to study improvements in the methods for isolation and storage of the heart.

Series 1.—  A bilateral anterior thoracotomy was performed and both sides of the heart of the donor animal were perfused with a variety of cold (4 C) electrolyte containing solutions after ligation of the vena cava. All vessels were then ligated and divided and the heart

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