April 25, 1966

Serum β-Glucuronidase in Diabetes Mellitus

Author Affiliations

Brockley Hill, Stanmore Middlesex, England

JAMA. 1966;196(4):373. doi:10.1001/jama.1966.03100170115048

To the Editor:—  In contrast to the findings of Miller et al (195:189, 1966) we found that the plasma β-glucuronidase (and N-acetyl-β-glucosaminidase) levels in diabetic subjects correlated significantly with the blood glucose concentration.1The discrepancy between our findings and those of Miller et al, and of Dohrmann2 is probably due to our use of platelet-free plasma in a sensitive fluorimetric assay procedure3 in contrast to the use of serum by other workers. It has been found that there is liberation of β-glucuronidase from the formed elements of blood during clotting, and the amount is sufficiently large and variable to obscure correlations such as those described. In this connection, we also found a correlation of β-glucuronidase activity with age; this was not observed by Miller et al or by previous workers.We believe that in atherosclerotic and nonatherosclerotic diabetic subjects, plasma β-glucuronidase, N-acetyl-β-glucosaminidase and blood glucose levels

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