The blister taken from the forearm revealed a subepidermal cleft with eosinophilic infiltration on histologic examination (Figure 2). On direct immunofluorescence, the skin specimen showed a linear deposition of IgG(Figure 3) and C3 along the basement membrane zone. Circulating anti–basement membrane zone IgG class antibodies were detected in a serum sample by indirect immunofluorescence using normal human skin. On indirect immunofluorescence of 1-mol/L sodium chloride split skin, the serum reacted with the epidermal side of the split. On Western immunoblot analysis of human epidermal extracts, the serum reacted with 230- and 180-kd bullous pemphigoid antigens (Figure 4).
Tense Blisters After Bone Marrow Transplantation. Arch Dermatol. 1999;135(1):81-c-86. doi:10-1001/pubs.Arch Dermatol.-ISSN-0003-987x-135-1-dof0199