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February 1999

Amplification of DNA of Borrelia burgdorferi in Urine Samples of Patients With Granuloma Annulare and Lichen Sclerosus et Atrophicus

Author Affiliations

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Arch Dermatol. 1999;135(2):210-212. doi:10-1001/pubs.Arch Dermatol.-ISSN-0003-987x-135-2-dlt0299

The detection of Borrelia burgdorferi DNA by polymerase chain reaction (PCR) analysis in urine samples has gained importance in the laboratory diagnosis of Lyme borreliosis. In the recent years, dermatoses, such as certain forms of morphea, Shulman syndrome, and, more recently, granuloma annulare (GA) and lichen sclerosus et atrophicus (LSA), have been linked with B burgdorferi infection.

In our study, a nested PCR method was used to investigate the urine samples of 13 patients with GA and 19 patients with LSA. Two different gene segments of B burgdorferi DNA were amplified, one derived from a specific part of the flagellin and the other from the chromosomal RNA polymerase C gene.

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