Copyright 2002 American Medical Association. All Rights Reserved. Applicable FARS/DFARS Restrictions Apply to Government Use.2002
Pseudallescheria boydii (anamorph: Scedosporium apiospermum) is a ubiquitous fungus that can cause opportunistic infections,1 and the polymerase chain reaction (PCR)–based method was recently developed for the identification of isolated fungi.2 We describe an immunocompromised patient with cutaneous infection by P boydii in whom seminested PCR with newly designed primers could detect the fungal DNA in preparations from paraffin-embedded sections.
Hagari Y, Ishioka S, Ohyama F, Mihara M. Cutaneous Infection Showing Sporotrichoid Spread Caused by Pseudallescheria boydii (Scedosporium apiospermum): Successful Detection of Fungal DNA in Formalin-Fixed, Paraffin-Embedded Sections by Seminested PCR. Arch Dermatol. 2002;138(2):271-272. doi:10.1001/archderm.138.2.266