In a recent article Reiss1 stated that blood serum taken from patients known to have lymphogranuloma inguinale produced an intradermal reaction comparable to that in the Frei test. The substitution of blood serum for Frei antigen would facilitate the more widespread use of intradermal tests for lymphogranuloma inguinale, which now frequently cannot be made because of the difficulty of obtaining Frei antigen.
A series of intradermal tests with blood serum was carried out in cases of recognized lymphogranuloma inguinale, in order to verify the results reported by Reiss.
Seven intradermal injections of 0.1 cc. each were made in the forearm of the only five patients with lymphogranuloma inguinale that were available. Each of these patients had previously shown a distinctly positive reaction to the Frei test. As control tests, similar injections were given to five patients having no clinical evidence of lymphogranuloma inguinale, each of whom
HAYNES HA. VALUE OF INTRADERMAL INJECTION OF SERUM AS A DIAGNOSTIC TEST FOR LYMPHO- GRANULOMA INGUINALE. Arch Derm Syphilol. 1935;32(5):795–797. doi:10.1001/archderm.1935.01470050091014