This paper deals with factors of importance in converting subsurface growths of pathogenic fungi into surface growths. It has a possible practical application, since substances which encourage surface growth when applied to the skin might attract organisms to the surface, where they would be more easily cultured or destroyed by antiseptics.
It was observed1 that a few millimeters of Sabouraud's proof medium poured over subsurface growths on cysteine mediums will bring them to the surface. Hydrolyzates of skin produced a growth which was on the surface and below it equally, while those of hair produced a growth almost entirely beneath the surface.2 Control growths in Sabouraud's proof medium were almost entirely on the surface. Representative amino acids gave predominantly subsurface growths.3 When cysteine was added to Sabouraud's proof medium, it failed to produce a growth noticeably more of which was subsurface.4 In all the mediums mentioned
WILLIAMS JW. PRODUCTION OF SURFACE GROWTH OF PATHOGENIC FUNGI ON CULTURE MEDIUMSSOME FACTORS OF IMPORTANCE. Arch Derm Syphilol. 1938;38(1):32–37. doi:10.1001/archderm.1938.01480130035005