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An Intradermal Reaction as an Aid in the Diagnosis of Granuloma Inguinale. Presented by Borris A. Kornblith, M.D.
A tissue antigen was prepared from an acute lesion of granuloma inguinale of six weeks' duration in which Donovan bodies were especially numerous and easily demonstrable on smear with Wright's stain (fig. 1 A). The extensive distribution of Donovan bodies in the resected specimen was easily demonstrated in paraffin tissue section stained by the Giemsa method.
The test material was prepared in the following manner: a fair-sized piece of tissue was removed and triturated in a small amount of isotonic solution of sodium chloride in a sterile mortar. The triturated material was diluted so that it would be possible to aspirate it through a 26 gage needle. It was then bottled and heated at 60 C. for two hours on one day and left in the refrigerator overnight. It was heated at
Bloom D, Lewis GM. NEW YORK ACADEMY OF MEDICINE, SECTION OF DERMATOLOGY AND SYPHILIS. Arch Derm Syphilol. 1944;50(4):274–283. doi:10.1001/archderm.1944.01510160046022