In a suspected case of chromoblastomycosis diagnosis can be confirmed by a simple office cultural study without the need for complex media.
With a ring curette tissue is scraped from the lesion until free bleeding occurs. This is then washed off in a small bottle containing about 5.0 ml. of distilled water and incubated at a minimum temperature of 35 C (room temperature).
In some three to five days blackish steel-gray nodular growths of the Fonsecaea (Hormodendrum) may be seen floating on the surface of the water. These are easily lifted from the surface, placed on a glass slide, and viewed microscopically without disturbing the mycelium.
143 Macquarie St.
HAVYATT M. Rapid Office Diagnosis of Chromoblastomycosis. AMA Arch Derm. 1957;75(6):850. doi:10.1001/archderm.1957.01550180064013