Methods of preparing intact, undenatured sheets of human stratum corneum are described. The epidermis of split or full-thickness skin is first separated by exposure to ammonia fumes or heat at 60 C. The horny layer is then isolated by incubating in dilute trypsin solution. Cantharidin blisters provide a convenient way of obtaining stratum corneum from living subjects with minimum discomfort and no scarring.
KLIGMAN AM, CHRISTOPHERS E. Preparation of Isolated Sheets of Human Stratum Corneum. Arch Dermatol. 1963;88(6):702-705. doi:10.1001/archderm.1963.01590240026005