A method is described by which the role of isohemagglutinins and heterohemagglutinins in the indirect basophil reaction has been studied.
Both isohemagglutinins and heterohemagglutinins systems with fresh human serum produced lysis of rabbit basophils. When guinea pig complement was added to the system there was an increase in the percentage lysis.
It is suggested that the method described in which the percentage of basophil lysis, rather than morphological changes, is considered as an end-point to the reaction may provide more easily readable and reproducible results for a qualitative and quantitative in vitro test.
Cohen HA. The Role of Hetero- and Isohemagglutinins in the Indirect Basophil Reaction. Arch Dermatol. 1967;95(3):313-318. doi:10.1001/archderm.1967.01600330071015