A psychiatric patient with a poison-ivy dermatitis was treated with chloride of lime and severe calcinosis cutis developed in the lesions. It was possible to reproduce these lesions of calcinosis in this patient. Calcium was identified both by laser spectroscopy and by histochemical studies. Because of the minute amount of tissue required for laser spectroscopy, biopsies both of lesions and of controls of normal skin were able to be processed for routine histological and histochemical studies after spectroscopy had been done on this same portion of tissue.
Wilson RG, Goldman L, Brech F. Calcinosis Cutis With Laser Microprobe AnalysisReport of a Self-Induced Case With Repeated Reproduction of Lesions. Arch Dermatol. 1967;95(5):490-495. doi:10.1001/archderm.1967.01600350052009