Sixty-three strains of Corynebacterium acnes (C acnes) were incubated individually in vitro with three representative triglycerides. Consistent differences were found in their ability to hydrolyze these triglycerides. Seventy-five percent of strains of C acnes isolated from patients with acne vulgaris split C12 triglyceride (trilaurin), 56% hydrolyzed C16 triglyceride (tripalmitin), and 88% cleaved C18:1 triglyceride (triolein). In comparison, 42% of isolates of C acnes from patients without acne vulgaris hydrolyzed C12 triglyceride (trilaurin), 17% of C acnes strains split C16 triglyceride (tripalmitin), and 58% split C18:1 triglyceride (triolein). The differences were significant to the 2% confidence level.
The results suggest that distinctive strains of C acnes inhabit the sebaceous follicles of the Tetracycline hydrochloride (10μg/ml and 30μg/ ml) blocked the in vitro hydrolyzing activity of six strains of C acnes known to possess such splitting abilities.
Kellum RE, Strangfeld K, Ray LF. Acne VulgarisStudies in Pathogenesis: Triglyceride Hydrolysis by Corynebacterium acnes in Vitro. Arch Dermatol. 1970;101(1):41-47. doi:10.1001/archderm.1970.04000010043006