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August 1970

Simplified Laboratory Technique for Diagnosis of Chancroid

Author Affiliations

San Francisco

From the Department of Clinical Microbiology (Dr. Borchardt) and the Dermatology Service (Dr. Hoke), US Public Health Service Hospital, San Francisco.

Arch Dermatol. 1970;102(2):188-192. doi:10.1001/archderm.1970.04000080060011

Presumptive microscopic identification of Haemophilus ducreyi can be made from clinical material obtained from a lesion. By employing 10 ml of patient's blood (inactivated at 56 C for 30 minutes) as a cultural medium and serous fluid from the lesion as an inoculum, confirmation may be completed within 48 hours. A total of 24 patients with presumptive diagnosis of chancroid were examined and 21 of these showed H ducreyi on smears and culture.