The concomitant application of tissue culture, phase contrast microscopy, and time-lapse cinematography offers enhanced opportunities for the study and possible identification of living cells. This union of techniques was applied to Kaposi's sarcoma, whose histogenesis remains uncertain a century after its first description. Explants were prepared from biopsy specimens of cutaneous lesions of Kaposi's sarcoma. Tissue cultures in Rose chambers were observed for two to six weeks. Four morphologically distinct cell types were characterized in these cultures, of which the most significant is the spindle-shaped cell. This cell exhibited aspects of the Schwann cell in culture.
Levan NE, Korn CS, Booher J, Rounds DE. Tissue Culture of Kaposi's Sarcoma. Arch Dermatol. 1972;106(1):37-40. doi:10.1001/archderm.1972.01620100025006