Methanol-fixed, paraffin-embedded bovine hoof or human heel sections have been used as substrates for the demonstration of antinuclear antibodies (ANA) by the indirect immunofluorescence technique. The method was compared to cryostat-cut mouse liver sections and was found to be as sensitive when using hoof sections and slightly less sensitive for human heel sections. The use of paraffin-embedded hoof sections makes the performance of the ANA test within reach of hospital laboratories that were previously unable to perform the procedure due to lack of equipment or manpower for cutting cryostat substrate sections.
Guss SB, Ugel AR. Methanol-Fixed Paraffin-Embedded Bovine and Human Epidermis for Antinuclear Antibodies. Arch Dermatol. 1972;106(5):674-677. doi:10.1001/archderm.1972.01620140022008