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April 1973

Phosphorylation in Mitochondria From Melanotic Melanoma

Author Affiliations

Beaverton, Ore

From the Department of Cutaneous Biology, Oregon Regional Primate Research Center, Beaverton. Dr. Kondo is now with the Max-Planck-Institute for Biophysics, Frankfurt, West Germany.

Arch Dermatol. 1973;107(4):583-586. doi:10.1001/archderm.1973.01620190059014

Mitochondria from B16 melanotic melanoma were isolated and their functional characteristics were studied.

Purified from the contaminating dark pigments by density-gradient ultracentrifugation, the mitochondria showed active oxidative phosphorylation, which was indicated by both normal adenosine diphosphate-oxygen ratios with intermediates of the tricarboxylic acid cycle and normal sensitivities to inhibitors and uncouplers.

The qualitative properties of mitochondria from melanotic mouse melanoma were essentially similar to those of mitochondria from amelanotic mouse melanoma and from normal, nonneoplastic tissues.