[Skip to Content]
Access to paid content on this site is currently suspended due to excessive activity being detected from your IP address 54.161.130.145. Please contact the publisher to request reinstatement.
[Skip to Content Landing]
Article
April 1973

Antinuclear Antibodies-Reply

Author Affiliations

Bethesda, MD

Arch Dermatol. 1973;107(4):631. doi:10.1001/archderm.1973.01620190095033
Abstract

To the Editor.—  In response to Dr. Burnham's letter, we quote a portion of our paper1:Although paraffin-embedded sections can not be used to differentiate most ANA patterns, paraffin-embedded sections can be used for screening patients and titering sera, thus, eliminating the need to make multiple cryostat-cut sections.If the above screening test is positive, the more precise cryostat methods should be used to determine which of the four nuclear patterns are present; if the pattern has been determined, the screening test can be used for following titers.The paraffin-embedded sections can be purchased and stored until needed, so that any hospital or interested investigator can perform the test. The shelf-life of the paraffinembedded material is at least five years. The use of paraffin-embedded material eliminates the routine need for fresh tumors, or laboratory animals, that are needed for preparing cryostat sections or imprints, which deteriorate within weeks

First Page Preview View Large
First page PDF preview
First page PDF preview
×