An in vitro method for monitoring a patient's response to therapy in disseminated coccidioidomycosis is presented. The procedure consists of direct microscopic examination for spherules and an evaluation of growth characteristics of the endospores in saline solution at timed intervals. The results correlated with both the skin and complement-fixation tests. This technique assists the clinician in measuring response to amphotericin B treatment and in determining when adequate therapy has been achieved.
Borchardt K, Litwack K, Hoke A, Imori M. In Vitro Monitoring of Amphotericin B Therapy in Disseminated Coccidioidomycosis. Arch Dermatol. 1973;108(1):119-120. doi:10.1001/archderm.1973.01620220081023