An in vivo skin perfusion technique was used to study the pharmacological activity in inflamed skin of patients with primary irritant dermatitis. Histamine was found in three and kinin activity in three of 13 patients. Pefusates from seven of the 13 patients contained a smooth muscle-contracting agent with prostaglandin-like properties. Solvent partition followed by thin-layer chromatography revealed this activity to be due to a mixture of prostaglandins E and F. This direct evidence supports the view that prostaglandins mediate sustained inflammation in man.
Søndergaard J, Greaves MW, Jørgensen HP. Recovery of Prostaglandins in Human Primary Irritant Dermatitis. Arch Dermatol. 1974;110(4):556-558. doi:10.1001/archderm.1974.01630100016003