The present study was designed to determine if lymphocytes from healthy humans could be stimulated to assume the morphologic appearance of Sézary-like cells.
Lymphocyte-rich populations of cells were incubated for 72 hours with pokeweed mitogen and phytohemagglutinin, both potent cellular mitogens. With light microscopy, differential cell counts performed on 0.5μ epoxy resin-embedded sections showed that 5% to 11% of the lymphocytes had cerebriform nuclei, and were designated as Sézary-like cells. The morphological results were confirmed by electron microscopy.
Production of Sézary-like cells from stimulated, normal, human lymphocytes suggests that cells with cerebriform nuclei may represent reactive lymphocytes, and may explain their presence in benign inflammatory dermatoses.
Yeckley JA, Weston WL, Thorne EG, Krueger GG. Production of Sézary-like Cells From Normal Human Lymphocytes. Arch Dermatol. 1975;111(1):29-32. doi:10.1001/archderm.1975.01630130031001