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September 1977

A Simple Permanent Mount of Sarcoptes scabiei

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Arch Dermatol. 1977;113(9):1296. doi:10.1001/archderm.1977.01640090144031

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To the Editor.—  In the current epidemic of scabies, more extensive efforts are being made to try to demonstrate the parasites. In some dermatological centers, at least in the past, it was required that to make a diagnosis of scabies the parasites had to be demonstrated. Most of the current techniques used for microscopic studies of skin scrapings are usually moist preparations. It is difficult to preserve such preparations for detailed search or for permanent preparations for records or for teaching purposes.A simple technique for preserving the parasite is to select what you consider the primary fresh lesion. This Permanent hair spray mount of Sarcoptes scabiei (May-Grünwald Giemsa stain, original magnification ×200). is scraped gently on a clean slide and immediately sprayed with hair spray such as is done for permanent preparations of Papanicolaou smears. May-Grünwald Giemsa stain may be used after this to help identify the parasites and

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