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To the editor.—
I have been very pleased with the following method of preparing a Tzanck test. The material to be tested is spread with a No. 15 blade on a regular glass slide. A small drop of methylene blue is applied and immediately covered with a coverslip. This is examined directly as a wet preparation under the microscope. The nuclear detail is sufficient to distinguish easily giant and multinucleated cells. The methylene blue is readily available from any histopathology laboratory.
Stocker WW. Preparing a Tzanck Test. Arch Dermatol. 1979;115(9):1033. doi:10.1001/archderm.1979.04010090001002