• In the present study we compared the accuracy of three different ultrastructural methods used to confirm clinically proved cases of mycosis fungoides (MF) from skin biopsy specimens. Method 1 is a qualitative method and confirmed the diagnosis of MF in only 44% of the patients with MF. Method 2 involved image analysis of lymphocytic nuclei and confirmed the diagnosis of MF in only 67% of the patients with MF. The most sensitive ultrastructural method for confirming the diagnosis of MF was a simple scoring of the number of sharply angled nuclear invaginations in 100 lymphocytes (method 3). Control biopsy specimens had many more lymphocytes (19% to 55%) with no sharply angled nuclear invaginations compared with those from patients with MF (3% to 15%). The clinical diagnosis of MF was confirmed in 100% of our patients with MF using method 3.
(Arch Dermatol 1984;120:63-75)
Payne CM, Nagle RB, Lynch PJ. Quantitative Electron Microscopy in the Diagnosis of Mycosis FungoidesA Simple Analysis of Lymphocytic Nuclear Convolutions. Arch Dermatol. 1984;120(1):63-75. doi:10.1001/archderm.1984.01650370069013