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—We read Dr Anderson's letter with interest, and appreciate his perceptive comments. We agree that in the analysis of fluorescence spectra of the skin, it is important to consider the effect of fluorescence absorption by endogenous compounds such as hemoglobin and melanin.While we agree that the "valley" between the recorded spectral peaks appears in the range of the absorption spectrum of hemoglobin, we would expect this effect to be more prominent rather than less prominent in photoaged skin that typically demonstrates telangiectasias. In addiNormal nontan human skin was tanned over a period of 7 days using natural sunlight. Sun-protected and sun-exposed areas were evaluated and readings were taken in each of four inner (nontan) and outer (tan) quadrants. tion, this "valley" was also noted in in vitro specimens, where boiling of the skin resulted in removal of collagen and, most likely, retained hemoglobin. We cannot appreciate any consistent
Leffell DJ, Stetz ML, Milstone L, Deckelbaum LI. In Vivo Fluorescence of Human Skin-Reply. Arch Dermatol. 1989;125(7):999-1000. doi:10.1001/archderm.1989.01670190133021