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February 1992


Author Affiliations

Departments of Dermatology and Pathology Massachusetts General Hospital Boston, MA 02114

Arch Dermatol. 1992;128(2):252-253. doi:10.1001/archderm.1992.01680120124017

Immunodermatopathology began with the description of the lupus band test by Burnham et al1 in 1963. The following year, Beutner and Jordon2 demonstrated the presence of autoantibodies in serum samples of patients with pemphigus vulgaris. Thus, the two most widely used techniques, direct and indirect immunofluorescence, were described very early in the development of the field. The other three generally available techniques include complement fixation indirect immunofluorescence (a method of amplification of the antigen staining that is most widely used in herpes gestationis), immunoelectron microscopy (generally restricted to research applications), and immunoperoxidase (most widely used for tumor antigen localization and for determining the anatomic level of bullous lesions in the skin). These immunologic diagnostic techniques have become the standard modalities of definitively confirming the diagnoses of antibody mediated (or associated) diseases. Research utilizing these antibodies and techniques continues to yield information regarding the pathophysiology of various diseases. The diagnostic

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