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May 1992

Treatment of Leg Ulcers With Cryopreserved Allogeneic Cultured EpitheliumA Multicenter Study

Author Affiliations

From the National Institute for Cancer Research, Laboratory of Cell Differentiation (Drs De Luca, Albanese, and Cancedda) and the General Hospital of Nervi (Dr Viacava and Faggioni), Genoa, Italy; and the Department of Dermatology, University of Modena (Italy) (Drs Zambruno and Giannetti).

Arch Dermatol. 1992;128(5):633-638. doi:10.1001/archderm.1992.01680150063007

• Background.—  In the past few years, several authors have described the usefulness of cultured allogeneic epidermal sheets in promoting wound healing of burns, leg ulcers, and donor sites. This study reports clinical results obtained by different departments in the treatment of chronic leg ulcers by cryopreserved cultured allogeneic epithelium. The freezing procedure and the assessment of viability of the cryopreserved epithelium are also described. A total of 30 ulcers were treated using 138 cryopreserved allografts.

Observations.—  Twenty ulcers (66.6%) healed completely within 12 weeks. Four ulcers showed a 30% to 84.4% reduction in size by 3 weeks but did not heal completely; the remaining six ulcers did not show any improvement. A strong stimulation of granulation tissue formation and of reepithelialization from the wound edge were observed.

Results.—  The results indicate that frozen cultured epidermis, stored in a skin bank, is a valid and generally applicable alternative therapy for the treatment of chronic ulcers.(Arch Dermatol. 1992;128:633-638)