Recently, Chang et al1 isolated DNA sequences from a new herpesvirus, human herpesvirus 8 (HHV-8), from Kaposi sarcoma (KS) specimens. Using polymerase chain reaction (PCR) with the KS330233 primers, Rady et al2 detected 233—base pair products in various epithelial skin tumors from 4 immunosuppressed patients. Boshoff et al3 did not confirm these data in 37 squamous cell carcinoma specimens from 25 immunosuppressed and 6 immunocompetent individuals using nested PCR with 2 sets of primers derived from the ORF26 gene and the conserved HHV-8 major capsid protein gene, ORF25. Therefore, further data are needed concerning these controversial findings on the presence of HHV-8 in lesions other than KS.
We have investigated the presence of HHV-8 sequences in 12 squamous cell carcinoma specimens, 6 basal cell carcinoma specimens, and 2 actinic keratosis specimens from 17 different immunosuppressed patients receiving transplants. The DNA was extracted from paraffin wax—embedded tissues,
Lebbé C, Tatoud R, Morel P, Calvo F, Euvrard S, Kanitakis J, Faure M, Claudy A. Human Herpesvirus 8 Sequences Are Not Detected in Epithelial Tumors From Patients Receiving Transplants. Arch Dermatol. 1997;133(1):111. doi:10.1001/archderm.1997.03890370123028