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Article
April 1997

Detection of Mycobacterium tuberculosis in Erythema Induratum of Bazin Using Polymerase Chain Reaction

Author Affiliations

Galveston

Mexico City, Mexico

University of Texas Medical Branch at Galveston 301 University Blvd Galveston, TX 77555-0783

Arch Dermatol. 1997;133(4):532-533. doi:10.1001/archderm.1997.03890400138029
Abstract

Tuberculosis poses a serious diagnostic and therapeutic problem, even in developed countries. As originally described,1 tuberculids are cutaneous eruptions that result from an abnormally marked host response to the presence of mycobacteria. One such tuberculid is erythema induratum of Bazin (EIB), in which Mycobacterium tuberculosis has recently been detected.2

Patients and Methods.  Sixteen formaldehyde solution— fixed, paraffin-embedded blocks of EIB were collected from 1988 to 1995 (Table).3 As a positive control in all procedures, a paraffin-embedded sample of M tuberculosis as confirmed by mycobacterial culture was included. As a negative control, an acrochordon specimen was included. Reagent controls were also included.DNA was extracted from paraffin-embedded blocks as previously described4 and analyzed for M tuberculosis DNA by polymerase chain reaction (PCR) using a primer specific for M tuberculosis and M bovis.5 The methods outlined by Eisenach et al5 were adhered to stringently with 1

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