Tuberculosis poses a serious diagnostic and therapeutic problem, even in developed countries. As originally described,1 tuberculids are cutaneous eruptions that result from an abnormally marked host response to the presence of mycobacteria. One such tuberculid is erythema induratum of Bazin (EIB), in which Mycobacterium tuberculosis has recently been detected.2
Patients and Methods.
Sixteen formaldehyde solution— fixed, paraffin-embedded blocks of EIB were collected from 1988 to 1995 (Table).3 As a positive control in all procedures, a paraffin-embedded sample of M tuberculosis as confirmed by mycobacterial culture was included. As a negative control, an acrochordon specimen was included. Reagent controls were also included.DNA was extracted from paraffin-embedded blocks as previously described4 and analyzed for M tuberculosis DNA by polymerase chain reaction (PCR) using a primer specific for M tuberculosis and M bovis.5 The methods outlined by Eisenach et al5 were adhered to stringently with 1
Yen A, Rady PL, Cortes-Franco R, Tyring SK. Detection of Mycobacterium tuberculosis in Erythema Induratum of Bazin Using Polymerase Chain Reaction. Arch Dermatol. 1997;133(4):532-533. doi:10.1001/archderm.1997.03890400138029