[Skip to Content]
Access to paid content on this site is currently suspended due to excessive activity being detected from your IP address 54.205.176.107. Please contact the publisher to request reinstatement.
[Skip to Content Landing]
Article
May 1997

Analysis of Tumor-Infiltrating Lymphocytes in Cutaneous Squamous Cell Carcinoma

Author Affiliations

From the Departments of Dermatology (Drs Haeffner, Zepter, Elmets, and Wood) and Pathology (Dr Wood), and the Skin Diseases Research Center (Drs Haeffner, Zepter, Elmets, and Wood), Case Western Reserve University, and the Veterans Affairs Medical Center (Dr Wood), Cleveland, Ohio. Dr Haeffner is now affiliated with the Department of Dermatology, University of Zurich, Zurich, Switzerland. Dr Zepter is now with the Department of Dermatology, University of Aachen, Aachen, Germany.

Arch Dermatol. 1997;133(5):585-590. doi:10.1001/archderm.1997.03890410039005
Abstract

Objective:  To characterize tumor-infiltrating lymphocytes (TILs) within lesions of cutaneous squamous cell carcinoma (SCC) and related disorders.

Design:  Case series with 1- and 2-color immunohistologic, molecular biological analysis of T-cell clonality and in vitro cytotoxicity assays.

Setting:  Academic medical center.

Patients:  Twenty-one patients, including 6 with actinic keratoses, 4 with SCC in situ, and 11 with invasive SCC.

Results:  CD8+ TILs were present within lesions of cutaneous SCC and AK. These cells constituted a variable minority of the total T-cell infiltrate, and many expressed a phenotype consistent with major histocompatibility complex—restricted cytotoxic T lymphocytes: CD3+, TIA1+, CD16-, CD56-, CD57-. They also expressed HLA-DR, suggesting their activation in vivo. Virtually all T cells were T-cell receptor (TCR)-β+δ-, indicating that they expressed the TCR-αβ protein heterodimer. Molecular biological analysis of TCR-γ gene rearrangements by the polymerase chain reaction and denaturing gradient gel electrophoresis technique indicated that the TILs were polyclonal. Functional studies suggested that TILs derived from SCC lesions were cytotoxic for autologous tumor cell targets.

Conclusion:  Tumor-infiltrating lymphocytes within cutaneous SCC lesions contain a subpopulation of polyclonal, major histocompatibility complex—restricted cytotoxic T lymphocytes expressing the TCR-αβ heterodimer.Arch Dermatol. 1997;133:585-590

×