We previously demonstrated a clonal dominance in the Vβ13.1 messages isolated from the lesional CD8+ T cells of psoriasis vulgaris, which suggested an interaction of Vβ13.1+ CD8+ T cells with skin antigens.
To determine whether the clonality observed accurately reflected a clonal population of infiltrating T cells or was skewed by an overabundance of messages from a small number of cells, and to extend our study of Vβ gene usage by lesional CD8+ T cells to 9 new patients.
Patients were enrolled at the Psoriasis Research Institute in Palo Alto, Calif, and samples were analyzed at The Immune Response Corporation in Carlsbad, Calif.
Main Outcome Measures:
For the 2 previous patients, skin samples were sorted directly for Vβ13.1+ T cells, for which the T-cell receptors were sequenced. For the 9 new patients, CD8+ T cells were sorted and their T-cell receptor Vβ gene usage measured using semiquantitative polymerase chain reaction with Vβ-specific primers.
The directly sorted Vβ13.1+ T cells exhibited clonal dominance in both patients. The dominant Vβ13.1 clone in each patient was the same as that found in the previous 2 biopsy specimens for which CD8+ T cells were sorted. Additionally, in 8 of the 9 new patients examined, we again found a preferential usage of Vβ3 and/or Vβ13.1 genes by the lesional CD8+ T cells.
The clonality, which was found in the Vβ messages of the sorted CD8+ T cells, accurately reflects the dominance of these clones in the infiltrating T cells. Moreover, the persistence in the same patient of the same clone for as long as 15 months and the overrepresentation of Vβ3 and/or Vβ13.1 in lesional CD8+ T cells in the new patients examined support the pathogenic role of T cells bearing these Vβs.Arch Dermatol. 1997;133:703-708
Chang JCC, Smith LR, Froning KJ, Kurland HH, Schwabe BJ, Blumeyer KK, Karasek MA, Wilkinson DI, Farber EM, Carlo DJ, Brostoff SW. Persistence of T-Cell Clones in Psoriatic Lesions. Arch Dermatol. 1997;133(6):703-708. doi:10.1001/archderm.1997.03890420031004