[Skip to Content]
Access to paid content on this site is currently suspended due to excessive activity being detected from your IP address 54.166.74.94. Please contact the publisher to request reinstatement.
[Skip to Content Landing]
Observation
September 2006

Granulomatous Skin Infection Caused by Malassezia pachydermatis in a Dog Owner

Author Affiliations

Author Affiliations: Department of Dermatology, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, China.

Arch Dermatol. 2006;142(9):1181-1184. doi:10.1001/archderm.142.9.1181
Abstract

Background  Malassezia pachydermatis is part of the normal cutaneous microflora of dogs and many other mammals. M pachydermatis has not yet been reported as an agent that causes skin infection in humans, although it has been found to cause fungemia and other nosocomial infections in preterm newborns and immunocompromised adults.

Observations  Malassezia pachydermatis was isolated from the facial granuloma of a healthy woman and her dog's skin scrapings and cerumen. The yeast identity was established by standard methods and scanning electron microscopy. A skin biopsy specimen showed chronic inflammatory granuloma, numerous purple-red round or ovoid spores in the superficial necrotic tissue, and sparse red spores in the dermis. The skin lesions healed after oral fluconazole and cryotherapy.

Conclusions  Definite diagnosis of M pachydermatis–induced skin infection principally depends on the results of fungal culture and histologic examination, and the combination of oral fluconazole and adjunctive cryotherapy seems to be an effective therapeutic regimen.

The genus Malassezia, comprising 10 distinct species, is principally recovered from the skin of mammals and birds but seldom from the environment.1,2Malassezia pachydermatis, Malassezia furfur, Malassezia globosa, and Malassezia sympodialis are generally considered to be the main species associated with clinical diseases.1Malassezia pachydermatis, the only non–lipid-dependent species of the genus Malassezia, was first isolated from the scales of an Indian rhinoceros (Rhinoceros unicornis) with exfoliative dermatitis by F. D. Weidman in 1925 and named Pityrosporum pachydermatis. With the synonymy of Malassezia (proposed by H. Baillon in 1889) and Pityrosporum (proposed by R. Sabouraud in 1904) being increasingly recognized and accepted in 1984 with anteriority for the generic Malassezia, P pachydermatis was then adopted as M pachydermatis, a name first introduced by C. W. Dodge in 1935 and accepted by M. A. Gordon in 1976.2 The importance of M pachydermatis has been recognized in both veterinary and human medicine.2 Skin colonization by M pachydermatis is frequent in wild and domestic carnivores, including dogs, cats, bears, ferrets, and foxes; less frequent in rhinoceros, pigs, primates, pinnipeds, horses, and birds; and undetected in rodents and lagomorphs.1,2 Human skin is commonly colonized by lipid-dependent Malassezia yeasts but rarely by M pachydermatis.3Malassezia pachydermatis has not yet been reported as an agent that causes skin infection, although it has been found to cause fungemia and other nosocomial infections in preterm newborns and immunocompromised adults.2,47 We isolated a strain of M pachydermatis from an immunocompetent woman with facial granuloma in April 2004. To our knowledge, this is the first report of M pachydermatis–induced skin infection in humans.

REPORT OF A CASE

A 46-year-old woman presented with an asymptomatic papule on her face in January 2004. The lesion enlarged gradually and appeared erosive and exudative after self-treatment with topical application of medicinal herbs. A similar lesion occurred on the left ala nasi 2 months later. She came to the Department of Dermatology at the Affiliated Hospital of Guangdong Medical College in April 2004. She had no history of local trauma and had kept a pet dog for 9 months. At examination, there was a painless verrucous plaque (5.2 × 3.1 cm) on the right side of her face that was covered with black and greasy crusts and surrounded by 2 nodules, and a yellowish hemispheroid nodule (0.5 cm in diameter) on the left ala nasi (Figure 1A). Local lymphadenopathy was absent.

Figure 1.
Patient before and after treatment. A, A verrucous plaque on the right side of the face and a hemispheroid nodule on the left ala nasi. B, After treatment, hypopigmented scar on the right side of the face.

Patient before and after treatment. A, A verrucous plaque on the right side of the face and a hemispheroid nodule on the left ala nasi. B, After treatment, hypopigmented scar on the right side of the face.

Potassium hydroxide preparation from skin lesions showed no fungal elements, but gram staining revealed numerous gram-positive, yeastlike polymorphous spores (Figure 2). A biopsy specimen showed chronic inflammatory granuloma. Epidermal hyperkeratosis, acanthosis, and obvious follicular dilation were apparent, with microabscesses composed of neutrophils in some of the hair follicles. The dermal inflammation was characterized by diffuse infiltration of primarily lymphocytes, plasmocytes, and histiocytes, with occasional eosinophils, neutrophils, and multinucleated giant cells. Periodic acid–Schiff (PAS) staining revealed numerous purple-red round or ovoid spores in the superficial necrotic tissue and sparse red spores in the dermis (Figure 3). The lesional secretions and the dog's skin scrapings and cerumen were cultured using Sabouraud dextrose agar (SDA) at 27°C, and yeastlike milky colonies grew on SDA supplemented with olive oil at 2 weeks and on SDA without lipid supplement at 3 weeks. The colony surface was matte, convex, and wrinkled, and the undersurface was flat. The surface color was ivory at first and darkened from yellow to brown with age. A direct smear showed small and less refractive yeast cells and spores. The fungus grew well on oily SDA at 27°C and 37°C, grew poorly at 41°C, and did not grow at 4°C or 8°C. Catalase test results were negative.

Figure 2.
Secretion smear showing numerous gram-positive, yeastlike polymorphous spores (gram stain; original magnification ×1000).

Secretion smear showing numerous gram-positive, yeastlike polymorphous spores (gram stain; original magnification ×1000).

Figure 3.
Biopsy specimen showing purple-red round or ovoid spores in the superficial necrotic tissue (periodic acid–Schiff stain; original magnification ×1000).

Biopsy specimen showing purple-red round or ovoid spores in the superficial necrotic tissue (periodic acid–Schiff stain; original magnification ×1000).

Scanning electron microscopy (Philips XL30; Philips Holland Eindhoven, the Netherlands) revealed that the cells were globose, ovoid, ellipsoidal, or cylindrical in shape and 2.35 to 2.6 μm × 2.07 to 2.1 μm in size. Unipolar blastic development was observed (eg, the daughter cell was separating from the cell wall of the mother cell at one end). The bud body was 1.39 × 1.37 μm in size, the collarette was 1.3 μm in breadth, and the base was circular and 0.36 μm thick. The characteristics of these colonies were compatible with those of M pachydermatis. Purified tuberculin test results were strongly positive (ie, vesiculation at the inoculation site at 72 hours). Other laboratory investigations did not reveal extracutaneous disease or immunosuppression.

The patient received 4 antituberculotics (isoniazid, rifampicin, pyrazinamide, and streptomycin sulfate) for 2 months because the results of direct examination and fungal culture using SDA without lipids were negative and the skin lesions were still augmented. According to positive findings of fungal culture and PAS staining, she was then treated with itraconazole (0.2 g/d), 10% potassium iodide solution (30 mL/d), and ciprofloxacin (0.4 g/d) for 2 weeks. Although the lesions stopped enlarging, the results of direct examination and fungal culture were still positive. Finally, she received fluconazole (0.2 g/d) for 10 weeks, ciprofloxacin (0.4 g/d) for 1 week, and liquid nitrogen cryotherapy 5 times. The skin lesions completely disappeared, leaving hypopigmented scars (Figure 1B). No relapse has occurred at 15 months of follow-up.

COMMENT

Malassezia pachydermatis is part of the normal cutaneous microflora of dogs and many other mammals.1Malassezia pachydermatis was first believed to be the pathogen of otitis externa in the dog by B. A. Gustafson in 1955 and the cause of canine chronic dermatitis by R. Dufait in 1983.2 In view of its importance as a canine pathogen, the carriage of M pachydermatis in dogs has been widely surveyed. Low numbers of M pachydermatis organisms colonize the stratum corneum in dogs with healthy skin, but their numbers may remarkably increase on the skin and within the ear canals in dogs with allergic skin diseases.1 However, few studies have examined the prevalence of M pachydermatis carriage in humans, although the potential exposure of human beings to the organism is great, especially in those keeping pets.1 In 200 healthy subjects, 24 (12%) were found to have low numbers of M pachydermatis on the scalp and palms according to fungal culture.3 Meanwhile, in another study, M pachydermatis was present on the skin of less than 1% of healthy volunteers and approximately 2% of patients with dermatitis who underwent fungal culture.8 The positive rates of M pachydermatis according to fungal culture and nested polymerase chain reaction were 6% and 92% in skin samples of 50 owners of healthy dogs and 38.7% and 93.3% in 50 owners of atopic dogs, respectively, indicating that the transfer of M pachydermatis from the canine skin to the human skin was frequent.1

Although M pachydermatis was isolated from the lacrimal duct in a 61-year-old man with canaliculitis, a skin wound in a 67-year-old man, and the urine in a patient with chronic granulomatous disease, the most reported cases are in preterm infants with intravascular catheter-acquired sepsis.2,4,5 In an intensive care nursery, positive culture results for M pachydermatis occurred in 15 low-birth-weight infants.6 In a report of fungemia in a neonatal intensive care unit (NICU), M pachydermatis infection and colonization appeared in 8 infants with very low birth weights who had various underlying diseases during a 6-month period.7 In addition, the source of the outbreak caused by M pachydermatis and its prophylactic measures have been investigated. The clinical isolates of M pachydermatis in an NICU were nosocomial for all strains isolated, with both patients and incubator surfaces being genetically indistinguishable; regular hygienic measures cannot adequately remove or kill the yeasts, which may persist on glass surfaces for at least 2 months despite regular cleaning of the incubators.9 However, M pachydermatis was likely introduced into the NICU from health care workers' hands after being colonized from pet dogs at home; careful hand washing by health care workers before and after contact with patients can effectively prevent the introduction and nosocomial transmission of the pet-associated yeast, since all cultures from the nursing staff and attending physicians were negative for the organism after hand-washing practices had been improved.6

Lipid supplementation is not an absolute requirement for the growth of M pachydermatis, but the addition of lipid material to the culture medium can enhance its growth.1,2 It grows at temperatures from 25°C to 41°C and seems to be sensitive to the cold.2Malassezia pachydermatis is characterized by cream-colored colonies with dry and smooth surfaces and short ovoid to ellipsoidal cells.2 The mode of conidium ontogeny was unipolar budding on a broad base, with a collarette.10Malassazia pachydermatis is easily identified by the colonies' morphologic and growth features and by microscopic examination, but our patient was initially misdiagnosed owing to the small and less refractive yeast cells on the potassium hydroxide preparation. These yeast cells are found only by careful high-powered microscopy in PAS-stained samples because of limited numbers of small and atypical spores, although a low-powered histologic view is useful to appreciate the pattern of inflammation. Therefore, definite diagnosis of M pachydermatis–induced skin infection mainly depends on fungal culture and histologic examination. Of course, the infection source should be carefully traced, and the dog's scurf and cerumen should undergo mycologic examination if possible. We speculate that the strain isolated from the patient could be the same as that from the dog's skin scrapings because of the regular mycologic examination results and the patient's close contact with the dog, although dog-to-patient transmission of M pachydermatis is unlikely to be proved by molecular differentiation because the isolate is nonviable.

In vitro susceptibility testing showed that M pachydermatis was sensitive to ketoconazole, itraconazole, and voriconazole.11 All isolated strains in an NICU were susceptible to amphotericin B, fluconazole, and itraconazole but resistant against flucytosine.7 Both pulse administration and once-daily administration of itraconazole were found to have similar effects in the treatment of canine cutaneous infection caused by M pachydermatis.12 Our patient achieved good results with combined treatment with oral fluconazole and cryosurgery, indicating that the combination of oral fluconazole and adjunctive cryotherapy is effective in the treatment of skin infection due to M pachydermatis and that the effectiveness of cryotherapy may be related to the vegetative character of the yeast (eg, no growth at 8°C or less).

Back to top
Article Information

Correspondence: Yi-Ming Fan, MD, Department of Dermatology, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, China 524001 (ymfan1963@163.com).

Financial Disclosure: None reported.

Accepted for Publication: March 14, 2006.

Author Contributions:Study concept and design: Fan. Acquisition of data: Fan, Huang, Li, Wu, Lai, and Chen. Analysis and interpretation of data: Fan and Huang. Drafting of the manuscript: Fan, Huang, Lai, and Chen. Critical revision of the manuscript for important intellectual content: Fan, Li, and Wu. Administrative, technical, and material support: Fan, Huang, and Wu. Study supervision: Fan and Li.

References
1.
Morris  DOO'Shea  KShofer  FSRankin  S Malassezia pachydermatis carriage in dog owners. Emerg Infect Dis 2005;1183- 88
PubMedArticle
2.
Guillot  JBond  R Malassezia pachydermatis: a review. Med Mycol 1999;37295- 306
PubMedArticle
3.
Bandhaya  M The distribution of Malassezia furfur and Malassezia pachydermatis on normal human skin. Southeast Asian J Trop Med Public Health 1993;24343- 346
PubMed
4.
Marcon  MJPowell  DA Human infections due to Malassezia spp. Clin Microbiol Rev 1992;5101- 119
PubMed
5.
Welbel  SFMcNeil  MMPramanik  A  et al.  Nosocomial Malassezia pachydermatis bloodstream infections in a neonatal intensive care unit. Pediatr Infect Dis J 1994;13104- 108
PubMedArticle
6.
Chang  HJMiller  HLWatkins  N  et al.  An epidemic of Malassezia pachydermatis in an intensive care nursery associated with colonization of health care workers' pet dogs. N Engl J Med 1998;338706- 711
PubMedArticle
7.
Chryssanthou  EBroberger  UPetrini  B Malassezia pachydermatis fungaemia in a neonatal intensive care unit. Acta Paediatr 2001;90323- 327
PubMedArticle
8.
Nakabayashi  ASei  YGuillot  J Identification of Malassezia species isolated from patients with seborrhoeic dermatitis, atopic dermatitis, pityriasis versicolor and normal subjects. Med Mycol 2000;38337- 341
PubMedArticle
9.
van Belkum  ABoekhout  TBosboom  R Monitoring spread of Malassezia infections in a neonatal intensive care unit by PCR-mediated genetic typing. J Clin Microbiol 1994;322528- 2532
PubMed
10.
Nishimura  KAsada  YTanaka  SWatanabe  S Ultrastructure of budding process of Malassezia pachydermatis. J Med Vet Mycol 1991;29387- 393
PubMedArticle
11.
Gupta  AKKohli  YLi  AFaergemann  JSummerbell  RC In vitro susceptibility of the seven Malassezia species to ketoconazole, voriconazole, itraconazole and terbinafine. Br J Dermatol 2000;142758- 765
PubMedArticle
12.
Pinchbeck  LRHillier  AKowalski  JJKwochka  KW Comparison of pulse administration versus once daily administration of itraconazole for the treatment of Malassezia pachydermatis dermatitis and otitis in dogs. J Am Vet Med Assoc 2002;2201807- 1812
PubMedArticle
×