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Figure 1.
Clinical pictures of herpetiform pemphigus (sample 6 in Table 1). Left, Multiple erythematous, urticarial plaques on the back. Right, Close-up of vesicles in a herpetiform arrangement on erythema of the arm.

Clinical pictures of herpetiform pemphigus (sample 6 in Table 1). Left, Multiple erythematous, urticarial plaques on the back. Right, Close-up of vesicles in a herpetiform arrangement on erythema of the arm.

Figure 2.
Immunoadsorption assays with recombinant desmoglein (rDsg) 1 and rDsg3 baculoproteins. A herpetiform pemphigus serum sample (sample 2 in Table 1) was incubated with control solution without baculoproteins (top) or a mixture of rDsg1 and rDsg3 baculoproteins (bottom) and subjected to immunofluorescence staining on healthy human skin. Immunoreactivity of the herpetiform pemphigus serum sample against keratinocyte cell surfaces was removed by incubation with a mixture of rDsg1 and rDsg3. Results are given at 1:40 dilution of the patient's serum sample. Bar represents 50 µm.

Immunoadsorption assays with recombinant desmoglein (rDsg) 1 and rDsg3 baculoproteins. A herpetiform pemphigus serum sample (sample 2 in Table 1) was incubated with control solution without baculoproteins (top) or a mixture of rDsg1 and rDsg3 baculoproteins (bottom) and subjected to immunofluorescence staining on healthy human skin. Immunoreactivity of the herpetiform pemphigus serum sample against keratinocyte cell surfaces was removed by incubation with a mixture of rDsg1 and rDsg3. Results are given at 1:40 dilution of the patient's serum sample. Bar represents 50 µm.

Immunologic Characteristics of Sera of Patients With Herpetiform Pemphigus*
Immunologic Characteristics of Sera of Patients With Herpetiform Pemphigus*
1.
Stanley  JR Cell adhesion molecules as targets of autoantibodies in pemphigus and pemphigoid, bullous diseases due to defective epidermal cell adhesion. Adv Immunol. 1993;53291- 325
2.
Amagai  MKlaus-Kovtun  VStanley  JR Autoantibodies against a novel epithelial cadherin in pemphigus vulgaris, a disease of cell adhesion. Cell. 1991;67869- 877Article
3.
Amagai  M Pemphigus: autoimmunity to epidermal cell adhesion molecules. Adv Dermatol. 1996;11319- 352
4.
Jablonska  SChorzelski  TPBeutner  EHChorselska  J Herpetiform pemphigus, a variable pattern of pemphigus. Int J Dermatol. 1975;14353- 359Article
5.
DeMento  FJGrover  RW Acantholytic herpetiform dermatitis. Arch Dermatol. 1973;107883- 887Article
6.
Seah  PPFry  LCairns  RJ  et al.  Pemphigus controlled by sulphapyridine. Br J Dermatol. 1973;8977- 81Article
7.
Barranco  VPOkla  T Mixed bullous disease. Arch Dermatol. 1974;110221- 224Article
8.
Maciejowska  EJablonska  SChorzelski  TP Is pemphigus herpetiformis an entity? Int J Dermatol. 1987;26571- 577Article
9.
Ingber  AFeuerman  EJ Pemphigus with characteristics of dermatitis herpetiformis: a long-term follow-up of five patients. Int J Dermatol. 1986;25575- 579Article
10.
Pye  RJ Bullous eruption. Champion  RHBurton  JLEbling  FJGeds.Textbook of Dermatology. 5th ed. Oxford, England Blackwell1992;1623- 1673
11.
Kinehara  MNishikawa  T A case of herpetiform pemphigus vulgaris. Jpn J Clin Dermatol. 1979;33495- 498
12.
Verdier  SSJoly  PThomine  E  et al.  Thiopronine-induced herpetiform pemphigus: report of a case studied by immunoelectron microscopy and immunoblot analysis. Br J Dermatol. 1994;130238- 240Article
13.
Shimizu  KHashimoto  TWang  N  et al.  A case of herpetiform pemphigus associated with autoimmune hemolytic anemia: detection of autoantibodies against multiple epidermal antigens. Dermatology. 1996;192179- 182Article
14.
Santi  CGMaruta  CWAoki  VSotto  MNRivitti  EADiaz  LA Pemphigus herpetiformis is a rare clinical expression of nonendemic pemphigus foliaceus, fogo selvagem, and pemphigus vulgaris. J Am Acad Dermatol. 1996;3440- 46Article
15.
Kubo  AAmagai  MHashimoto  T  et al.  Herpetiform pemphigus showing reactivity with pemphigus vulgaris antigen (desmoglein 3). Br J Dermatol. 1997;137109- 113Article
16.
Niizeki  HKonohana  IIkutomi  MMita  SHashimoto  T A case of herpetiform pemphigus. Jpn J Clin Dermatol. 1992;46831- 834
17.
Kikuchi  ASakuraoka  KHashimoto  TNishikawa  TShiotani  C A case of herpetiform pemphigus. Jpn J Clin Dermatol. 1992;131139- 1142
18.
Amagai  MHashimoto  TGreen  KJShimizu  NNishikawa  T Antigen-specific immunoadsorption of pathogenic autoantibodies in pemphigus foliaceus. J Invest Dermatol. 1995;104895- 901Article
19.
Amagai  MHashimoto  TShimizu  NNishikawa  T Absorption of pathogenic autoantibodies by the extracellular domain of pemphigus vulgaris antigen (Dsg3) produced by baculovirus. J Clin Invest. 1994;9459- 67Article
20.
Ishii  KAmagai  MHall  RP  et al.  Characterization of autoantibodies in pemphigus using antigen-specific enzyme-linked immunosorbent assays with baculovirus-expressed recombinant desmogleins. J Immunol. 1997;1592010- 2017
21.
Amagai  MKoch  PJNishikawa  TStanley  JR Pemphigus vulgaris antigen (desmoglein 3) is localized in the lower epidermis, the site of blister formation in patients. J Invest Dermatol. 1996;106351- 355Article
22.
Hashimoto  TOgawa  MMKonohana  ANishikawa  T Detection of pemphigus vulgaris and pemphigus foliaceus antigens by immunoblot analysis using different antigen sources. J Invest Dermatol. 1990;94327- 331Article
23.
Ishii  K Development of ELISAs with recombinant pemphigus antigens expressed by baculovirus. Jpn J Dermatol. 1998;108937- 942
24.
Hashimoto  TKiyokawa  CMori  O  et al.  Human desmocollin (Dsc1) is an autoantigen for the subcorneal pustular dermatosis type of IgA pemphigus. J Invest Dermatol. 1997;109127- 131Article
25.
Schiltz  JRMichel  B Production of epidermal acantholysis in normal human skin in vitro by the IgG fraction from pemphigus serum. J Invest Dermatol. 1976;67254- 260Article
26.
Hashimoto  KShafran  KMWebber  PSLazarus  GSSinger  KH Anti–cell surface pemphigus autoantibody stimulates plasminogen activator activity of human epidermal cells. J Exp Med. 1983;157259- 272Article
27.
Anhalt  GJLabib  RSVoorhees  JJBeals  TFDiaz  LA Induction of pemphigus in neonatal mice by passive transfer of IgG from patients with the disease. N Engl J Med. 1982;3061189- 1196Article
Study
August 1999

Desmoglein 1 and Desmoglein 3 Are the Target Autoantigens in Herpetiform Pemphigus

Author Affiliations

From the Departments of Dermatology, Keio University School of Medicine, Tokyo (Drs Ishii, Amagai, and Nishikawa), and Saiseikai Central Hospital, Tokyo (Dr Ebihara), the Department of Dermatology, Kurume University School of Medicine, Fukuoka (Drs Komai and Hashimoto), and Medical and Biological Laboratories, Nagoya (Mr Ohya), Japan; and the Department of Dermatology, Warsaw School of Medicine, Warsaw, Poland (Drs Chorzelski and Jablonska).

Arch Dermatol. 1999;135(8):943-947. doi:10.1001/archderm.135.8.943
Abstract

Objective  To determine the cell surface autoimmune target of herpetiform pemphigus (HP).

Design  Serum samples of HP were examined by immunoblot studies with human epidermal extracts, enzyme-linked immunosorbent assay with baculovirus-expressed recombinant desmoglein (rDsg) 1 and rDsg3, and immunoadsorption assay with rDsg.

Patients  Twenty serum samples were obtained from patients with HP who have typical clinical and histological features. All serum samples showed positive staining against keratinocyte cell surfaces by indirect immunofluorescence studies with healthy human skin.

Results  Immunoblot results showed that of 17 HP serum samples, only 5 reacted with a 160-kd band and 1 reacted with a 130-kd band. Results of enzyme-linked immunosorbent assays with rDsg1 and rDsg3 demonstrated that of 20 HP serum samples, 16 were positive against Dsg1 and 4 were positive against Dsg3. No serum samples reacted with both. Furthermore, in 19 of 20 HP serum samples, immunoreactivity against keratinocyte cell surfaces was completely removed by preincubation with rDsg1 and rDsg3 as shown by indirect immunofluorescence, excluding a possibility that these HP sera contain autoantibodies against other cell surface molecules.

Conclusions  Dsg1 and Dsg3 are the major cell surface target molecules of HP, suggesting that most cases of HP are clinical variants of pemphigus foliaceus and that the rest might be variants of pemphigus vulgaris.

PEMPHIGUS IS a group of antibody-mediated autoimmune bullous diseases. Classic pemphigus consists of 2 major subtypes—pemphigus foliaceus (PF) and pemphigus vulgaris (PV). By immunoblotting and immunoprecipitation, autoantigens of PF and PV have been demonstrated to be 160- and 130-kd glycoproteins, respectively.1 Results of complementary DNA cloning of both molecules revealed that the target molecules of PF and PV are desmoglein (Dsg) 1 and Dsg3, respectively, which locate in desmosomes.2,3

Herpetiform pemphigus (HP) was first introduced by Jablonska and colleagues4 as a variant of pemphigus for a patient with clinical features of dermatitis herpetiformis and immunologic features of pemphigus. Various terms have been used to describe this condition, such as acantholytic herpetiform dermatitis,5 pemphigus controlled by sulfapyridine,6 and mixed bullous disease.7 Clinical manifestations consist of erythematous, urticarial plaques and vesicles that present in herpetiform arrangement. Histological findings of HP are variable and include eosinophilic spongiosis and subcorneal pustules with minimal or no apparent acantholysis. Immunofluorescence findings show that most patients with HP have IgG antibodies against keratinocyte cell surfaces.8 Its position in the pemphigus group remains controversial. Based on its clinical features, some researchers8,9 reported the disease as a distinct entity and consider it to be different from classic pemphigus because of its clinical peculiarity and benign course. However, others described it as a variant of PF10 or PV.11

Several sporadic studies characterize the target antigens of HP by immunochemical analyses such as immunoblotting or immunoprecipitation. Some studies1214 claimed that HP sera recognized the 160-kd Dsg1, and another15 claimed that HP serum recognized the 130-kd Dsg3. Some authors16,17 found that HP sera did not react with any peptide in epidermal extracts by immunoblotting, although immunoblot does not detect conformational epitopes. Therefore, the target molecules of HP remain unclear.

Amagai et al18,19 previously produced recombinant Dsg 1 (rDsg1) and rDsg3 by baculovirus expression system, which properly reflects native conformational epitopes. Using these recombinant antigens, Ishii et al20 developed a sensitive and highly specific enzyme-linked immunosorbent assay (ELISA) system for detection of autoantibodies against Dsg1 and Dsg3. Amagai et al21 also developed an immunoadsorption assay using these recombinant proteins to determine whether there are antibodies against molecules other than Dsg1 or Dsg3.

The purpose of this study was to characterize the autoantigen of HP. As a target antigen, we focused on Dsg1 and Dsg3, which are the autoantigens of PF and PV, respectively. Serum samples from patients with HP were analyzed by immunoblotting, ELISA with rDsg1 and rDsg3, and immunoadsorption assay. We demonstrated that HP serum samples contain IgG against either Dsg1 or Dsg3 and that in most HP serum samples there were no antibodies directed against cell surface molecules other than Dsg1 or Dsg3.

MATERIALS AND METHODS
SERUM SAMPLES

Serum samples were obtained from 20 patients with HP whose clinical manifestations resemble those of dermatitis herpetiformis in the form of the eruption, its symmetrical distribution, and pruritus. It consists of erythematous papules, urticarial-like plaques, or vesicles. Typically, annular erythematous plaques with vesicles and erosions on their margins were seen on the trunk and extremities (Figure 1). Oral membrane lesions were involved in some patients. The Nikolsky sign was negative. Results of histological examination showed eosinophilic spongiosis and subcorneal pustules with minimal or no apparent acantholysis. Direct immunofluorescence stainings revealed the deposition of IgG on keratinocyte cell surfaces in all samples. We selected serum samples that showed positive staining against keratinocyte cell surfaces by indirect immunofluorescence testing using healthy human skin as a substrate.

IMMUNOBLOT ANALYSIS

Immunoblotting was performed as previously reported.22 EDTA-separated healthy human epidermal extracts were used as the source of antigens.

ELISA USING rDsg1 AND rDsg3

Ishii et al20 previously developed an ELISA system using baculovirus-expressed rDsg1 and rDsg3. Scores on ELISA against Dsg1 and Dsg3 were obtained as previously reported with a slight modification. Briefly, 201-fold diluted serum samples underwent reaction for 1 hour, and then peroxidase-conjugated antihuman IgG antibodies (Medical and Biological Laboratories, Nagoya, Japan) were used as a second antibody. Color development was achieved with the tetramethylbenzidine solution for 30 minutes and stopped by adding 2N sulfuric acid. The absorbance was measured at 450 nm by an ELISA reader (Bio-Rad Laboratories, Hercules, Calif).

In the previous ELISA, 3 serial dilutions of test serum samples and 8 serial dilutions of a standard serum sample had to be made each time to calculate reaction unit. In this study, we simplified the calculation procedure and used an index value. A single PF serum sample and PV serum sample were selected as positive control serum samples for Dsg1 and Dsg3 ELISA, respectively. The index was calculated as follows: index=(OD [optical density] of tested serum−OD of negative control)/(OD of positive control serum−OD of negative control)×100.

A cutoff value was defined as the average value +3 SDs of 47 control serum samples. Cutoff values of Dsg1 and Dsg3 ELISAs were defined as 14.1 and 15.4 index, respectively.23

To detect IgA-class autoantibodies against Dsg1 and Dsg3, we modified the ELISA by using peroxidase-conjugated antihuman IgA antibodies (Medical and Biological Laboratories) as second antibodies. Reactivity of IgA-class antibodies was indicated with OD and compared with those of control serum samples.

IMMUNOADSORPTION ASSAYS WITH rDsg1 AND rDsg3 BACULOPROTEINS

Serum samples of HP were serially diluted at 1:40 and 1:160 with a 1:1 mixture of culture supernatant of rDsg1 baculoprotein (Dsg1-His) and rDsg3 baculoprotein (Dsg3-His) and also with culture supernatant without rDsg baculoprotein as a control. After incubation at 4°C overnight, the diluted serum samples were subjected to indirect immunofluorescence on cryosectioned healthy human skin using 1:100 dilution of fluorescein isothiocyanate–conjugated antihuman IgG antibodies (Dako, Copenhagen, Denmark). The immunoreactivity against keratinocyte cell surfaces at 1:40 dilution of serum samples was judged for immunoadsorption assay. Negative immunoreactivity after preincubation with rDsg1 and rDsg3 indicates that the serum samples contain only Dsg3 or Dsg1 antibodies. When the immunoreactivity was not removed even after preincubation with rDsg1 and rDsg3, it indicates that they contain antibodies against the cytoplasmic domain of Dsg1 or Dsg3 or other components of keratinocyte cell surfaces.

RESULTS
ANALYSIS BY IMMUNOBLOTTING STUDIES

Seventeen HP serum samples were examined by immunoblotting studies using healthy human epidermal extracts. Five serum samples recognized a 160-kd polypeptide, which comigrated with the band recognized by PF sera. Only 1 serum sample recognized a 130-kd polypeptide, which comigrated with the band recognized by PV sera (Table 1).

ANALYSIS BY rDsg1 AND rDsg3 ELISAs

In immunoblotting, the antigen sources are denatured by heating and sodium dodecyl sulfate. Therefore, immunoblotting cannot detect autoantibodies against the conformationally sensitive epitope. In contrast, the ELISA system with rDsg1 and rDsg3 expressed by baculovirus detects autoantibodies against conformational epitopes on Dsg1 or Dsg3. Of 20 HP serum samples tested, 16 showed positive reactivity against Dsg1 ELISA, 4 showed positive reactivity against Dsg3 ELISA, and none reacted with both (Table 1).

Regarding IgA-class anti-Dsg1 and anti-Dsg3 antibodies, we analyzed these serum samples by ELISA. Four HP serum samples showed slightly higher OD values compared with those of control serum samples (samples 5 and 6 for Dsg1 ELISA and samples 18 and 19 for Dsg3 ELISA). However, the meaning of reactivities against Dsg1 and Dsg3 was unclear because indirect immunofluorescence showed no staining on keratinocyte cell surfaces for IgA antibodies (data not shown).

HP SERA DO NOT CONTAIN IgG AGAINST CELL SURFACE MOLECULES OTHER THAN Dsg1 OR Dsg3

To further determine whether HP serum samples possess antibodies against other components of keratinocyte cell surfaces, an immunoadsorption assay was performed. In 19 of 20 HP serum samples examined, immunoreactivity against keratinocyte cell surfaces was completely removed by preincubation with a mixture of rDsg3 and rDsg1 baculoproteins, whereas control medium did not change the immunoreactivity (Table 1 and Figure 2). This finding indicates that most HP serum samples possess only antibodies against the extracellular domain of Dsg1 or Dsg3 and no other antibodies against components of keratinocyte cell surfaces other than Dsg1 or Dsg3.

COMMENT

Herpetiform pemphigus is a unique form of pemphigus that clinically resembles dermatitis herpetiformis—an IgA-mediated disease—and immunologically has a feature of pemphigus—circulating and in vivo bound IgG autoantibodies against keratinocyte cell surfaces. To date, sporadic reports indicate that patients with HP have circulating autoantibodies against Dsg1 or Dsg3, as determined by immunoblotting or immunoprecipitation. However, a large-scale study has not been done to determine the autoimmune target for HP. In this study, we obtained serum samples from 20 patients with typical HP and analyzed them with ELISA and immunoadsorption assay with rDsg1 and rDsg3. The ELISA analysis demonstrated that of 20 HP serum samples, 16 had anti-Dsg1 IgG autoantibodies and 4 had anti-Dsg3 IgG autoantibodies. Furthermore, immunoadsorption study results excluded a possibility that there are any autoantibodies reacting with molecules on keratinocyte cell surfaces other than Dsg1 or Dsg3 in 19 of 20 HP serum samples. These findings indicate that Dsg1 is the most frequently recognized autoimmune target molecule in HP and that Dsg3 is the alternative autoimmune target molecule.

There was 1 HP serum sample that had anti-Dsg3 IgG autoantibodies but whose immunoreactivity was not removed by preincubation with a mixture of rDsg1 and rDsg3. This particular serum sample may contain autoantibodies against molecules other than Dsg3 in addition to antibodies against Dsg3. Alternatively, this sample may have autoantibodies against the cytoplasmic portion of Dsg1 or Dsg3 because the recombinant proteins used for ELISA and immunoadsorption assay represent only the extracellular domains. Immunofluorescence staining of this serum showed a unique pattern with coarse dotlike staining along the cell-cell contact sites (data not shown), which favors the former possibility. However, there are no specific clinical features in this case, and therefore the pathophysiological relevance of this antibody is currently unknown.

Herpetiform pemphigus has some clinical and histological features in common with IgA pemphigus. However, HP can be differentiated from IgA pemphigus by the presence of IgG-class antibodies against keratinocyte cell surfaces because IgA pemphigus is characterized by the presence of IgA-class antibodies without the coexistence of IgG-class antibodies against keratinocyte cell surfaces.24

A more intriguing question is why the clinical presentations of HP and classic pemphigus are so different despite the common presence of anti-Dsg1 or anti-Dsg3 IgG autoantibodies. Clinical pictures of HP consist of erythematous, urticarial plaques with occasional vesicles in herpetiform arrangement. Histological pictures of HP include eosinophilic spongiosis and subcorneal pustules with minimal or no apparent acantholysis, whereas, in PF and PV, acantholysis caused by loss of cell adhesion of keratinocytes is the major histological finding. The pathogenic role of anti-Dsg1 or anti-Dsg3 IgG autoantibodies in PF and PV is established. First, IgG fractions prepared from patients with PF and PV can induce blisters in skin organ culture and in neonatal mice.2527 Second, removal of anti-Dsg1 or anti-Dsg3 autoantibodies from PF or PV sera, respectively, abolished the ability of the sera to induce blister formation in neonatal mice.18,19 Third, Dsg1 and Dsg3 are expressed in the epidermis, where blister formation occurs in PF and PV, respectively.21 At present, the distinct pathophysiological events responsible for these differences are unknown. However, it can be speculated that the phenotype differences may be caused by differences of epitopes recognized by autoantibodies. Autoantibodies in PF and PV recognize functionally important regions on Dsg and inhibit their adhesive function, leading to the loss of cell adhesion. In contrast, autoantibodies in HP may recognize a functionally less important part of the molecule and therefore are not able to induce loss of cell adhesion. However, these antibodies may be sufficient to cause some inflammatory processes through complement or induction of cytokine release by keratinocytes, leading to intercellular edema and eosinophilic spongiosis.

Results of this study demonstrate that Dsg1 and Dsg3 are the target cell surface antigens of HP. Most HP serum samples contained the autoantibodies against Dsg1. The fact that several patients with HP show features of PF or PV in the course of the disease, or that some patients with HP evolved into having PF or PV, suggests that HP is closely related to PF and PV.8,14 Taking these together, we conclude that HP is a clinical and histological variant of PF or PV.

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Article Information

Reprints: Masayuki Amagai, MD, PhD, Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi Shinjuku-ku, Tokyo, 160-8582, Japan (e-mail: amagai@mc.med.keio.ac.jp).

Accepted for publication January 27, 1999.

This work was supported by a Grant-In-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan; a grant from the Ministry of Health and Welfare of Japan; Research Grants for Life Sciences and Medicine from the Keio University Medical Science Fund; and Keio Gijuku Academic Development Funds, Tokyo, Japan.

Presented in part at International Investigative Dermatology 1998, Cologne, Germany, May 10, 1998.

We thank Minae Suzuki for immunofluorescence staining.

References
1.
Stanley  JR Cell adhesion molecules as targets of autoantibodies in pemphigus and pemphigoid, bullous diseases due to defective epidermal cell adhesion. Adv Immunol. 1993;53291- 325
2.
Amagai  MKlaus-Kovtun  VStanley  JR Autoantibodies against a novel epithelial cadherin in pemphigus vulgaris, a disease of cell adhesion. Cell. 1991;67869- 877Article
3.
Amagai  M Pemphigus: autoimmunity to epidermal cell adhesion molecules. Adv Dermatol. 1996;11319- 352
4.
Jablonska  SChorzelski  TPBeutner  EHChorselska  J Herpetiform pemphigus, a variable pattern of pemphigus. Int J Dermatol. 1975;14353- 359Article
5.
DeMento  FJGrover  RW Acantholytic herpetiform dermatitis. Arch Dermatol. 1973;107883- 887Article
6.
Seah  PPFry  LCairns  RJ  et al.  Pemphigus controlled by sulphapyridine. Br J Dermatol. 1973;8977- 81Article
7.
Barranco  VPOkla  T Mixed bullous disease. Arch Dermatol. 1974;110221- 224Article
8.
Maciejowska  EJablonska  SChorzelski  TP Is pemphigus herpetiformis an entity? Int J Dermatol. 1987;26571- 577Article
9.
Ingber  AFeuerman  EJ Pemphigus with characteristics of dermatitis herpetiformis: a long-term follow-up of five patients. Int J Dermatol. 1986;25575- 579Article
10.
Pye  RJ Bullous eruption. Champion  RHBurton  JLEbling  FJGeds.Textbook of Dermatology. 5th ed. Oxford, England Blackwell1992;1623- 1673
11.
Kinehara  MNishikawa  T A case of herpetiform pemphigus vulgaris. Jpn J Clin Dermatol. 1979;33495- 498
12.
Verdier  SSJoly  PThomine  E  et al.  Thiopronine-induced herpetiform pemphigus: report of a case studied by immunoelectron microscopy and immunoblot analysis. Br J Dermatol. 1994;130238- 240Article
13.
Shimizu  KHashimoto  TWang  N  et al.  A case of herpetiform pemphigus associated with autoimmune hemolytic anemia: detection of autoantibodies against multiple epidermal antigens. Dermatology. 1996;192179- 182Article
14.
Santi  CGMaruta  CWAoki  VSotto  MNRivitti  EADiaz  LA Pemphigus herpetiformis is a rare clinical expression of nonendemic pemphigus foliaceus, fogo selvagem, and pemphigus vulgaris. J Am Acad Dermatol. 1996;3440- 46Article
15.
Kubo  AAmagai  MHashimoto  T  et al.  Herpetiform pemphigus showing reactivity with pemphigus vulgaris antigen (desmoglein 3). Br J Dermatol. 1997;137109- 113Article
16.
Niizeki  HKonohana  IIkutomi  MMita  SHashimoto  T A case of herpetiform pemphigus. Jpn J Clin Dermatol. 1992;46831- 834
17.
Kikuchi  ASakuraoka  KHashimoto  TNishikawa  TShiotani  C A case of herpetiform pemphigus. Jpn J Clin Dermatol. 1992;131139- 1142
18.
Amagai  MHashimoto  TGreen  KJShimizu  NNishikawa  T Antigen-specific immunoadsorption of pathogenic autoantibodies in pemphigus foliaceus. J Invest Dermatol. 1995;104895- 901Article
19.
Amagai  MHashimoto  TShimizu  NNishikawa  T Absorption of pathogenic autoantibodies by the extracellular domain of pemphigus vulgaris antigen (Dsg3) produced by baculovirus. J Clin Invest. 1994;9459- 67Article
20.
Ishii  KAmagai  MHall  RP  et al.  Characterization of autoantibodies in pemphigus using antigen-specific enzyme-linked immunosorbent assays with baculovirus-expressed recombinant desmogleins. J Immunol. 1997;1592010- 2017
21.
Amagai  MKoch  PJNishikawa  TStanley  JR Pemphigus vulgaris antigen (desmoglein 3) is localized in the lower epidermis, the site of blister formation in patients. J Invest Dermatol. 1996;106351- 355Article
22.
Hashimoto  TOgawa  MMKonohana  ANishikawa  T Detection of pemphigus vulgaris and pemphigus foliaceus antigens by immunoblot analysis using different antigen sources. J Invest Dermatol. 1990;94327- 331Article
23.
Ishii  K Development of ELISAs with recombinant pemphigus antigens expressed by baculovirus. Jpn J Dermatol. 1998;108937- 942
24.
Hashimoto  TKiyokawa  CMori  O  et al.  Human desmocollin (Dsc1) is an autoantigen for the subcorneal pustular dermatosis type of IgA pemphigus. J Invest Dermatol. 1997;109127- 131Article
25.
Schiltz  JRMichel  B Production of epidermal acantholysis in normal human skin in vitro by the IgG fraction from pemphigus serum. J Invest Dermatol. 1976;67254- 260Article
26.
Hashimoto  KShafran  KMWebber  PSLazarus  GSSinger  KH Anti–cell surface pemphigus autoantibody stimulates plasminogen activator activity of human epidermal cells. J Exp Med. 1983;157259- 272Article
27.
Anhalt  GJLabib  RSVoorhees  JJBeals  TFDiaz  LA Induction of pemphigus in neonatal mice by passive transfer of IgG from patients with the disease. N Engl J Med. 1982;3061189- 1196Article
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