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The Cutting Edge
September 2004

Low-Dose Tissue Plasminogen Activator for Calciphylaxis

Author Affiliations
 

GEORGE J.HRUZAMDMICHAEL P.HEFFERNANMDELAINESIEGFRIEDMD

Arch Dermatol. 2004;140(9):1045-1048. doi:10.1001/archderm.140.9.1045
REPORT OF A CASE

A 75-year-old man with end-stage renal disease presented with a 6-month history of nonhealing, markedly painful, necrotic ulcerations of the lower extremities (Figure 1A). The ulceration progressed in spite of multiple treatments elsewhere that included intensive wound care with wet-to-dry dressings, topical becaplermin, surgical debridement, and Unna boot dressings. A skin biopsy specimen demonstrated intravascular and extravascular calcification with pannicular necrosis and fibrin thrombi in dermal blood vessels consistent with calciphylaxis (Figure 2).

Figure 1.
A, Ulcers before treatment (note inflamed, necrotic ulceration on lower extremity). B, Appearance of ulcers immediately after treatment. C, Appearance of ulcers 6 months after treatment with tissue plasminogen activator.

A, Ulcers before treatment (note inflamed, necrotic ulceration on lower extremity). B, Appearance of ulcers immediately after treatment. C, Appearance of ulcers 6 months after treatment with tissue plasminogen activator.

Figure 2.
Histopathologic features of affected skin. A, Multifocal vascular and extravascular calcification with pannicular and epidermal necrosis (hematoxylin-eosin, original magnification ×25). B, Affected arteriole exhibiting mural calcification and intimal hyperplasia (hematoxylin-eosin, original magnification ×400). C, Fibrin thrombi within superficial dermal vessels (hematoxylin-eosin, original magnification ×400).

Histopathologic features of affected skin. A, Multifocal vascular and extravascular calcification with pannicular and epidermal necrosis (hematoxylin-eosin, original magnification ×25). B, Affected arteriole exhibiting mural calcification and intimal hyperplasia (hematoxylin-eosin, original magnification ×400). C, Fibrin thrombi within superficial dermal vessels (hematoxylin-eosin, original magnification ×400).

The patient had been receiving peritoneal dialysis for approximately 1 year for end-stage renal disease. His medical history included recurrent deep venous thrombosis, chronic active hepatitis C, obstructive sleep apnea, and hypertension. His serum albumin level was decreased at 1.9 g/dL (normal, 3.4-4.8 g/dL) and the serum calcium level was 9.1 mg/dL (2.3 mmol/L) (corrected, 10.8 mg/dL [2.7 mmol/L]; normal, 8.9-10.1 mg/dL [2.2-2.5 mmol/L]). The serum phosphorus and parathyroid hormone levels were increased at 5.2 mg/dL (1.7 mmol/L) (normal, 2.5-4.5 mg/dL [0.8-1.5 mmol/L]) and 8.5 pmol/L (normal, 1.0-5.2 pmol/L), respectively. The calcium-phosphate product was 47.2 mg2/dL2 (corrected, 56.0 mg2/dL2).

Coagulation screening tests demonstrated the following: low factor II activity at 35% (normal, 70%-130%), low factor VII activity at 48% (normal, 65%-140%), low antithrombin III antigen and activity level at 72% and 59%, respectively (normal, 84%-120% and 80%-120%, respectively), low protein C antigen and activity level at 42% and 30%, respectively (normal, 60%-125% and 70%-130%, respectively), a normal protein S antigen and activity level, and an increased level of fibrinogen at 671 mg/dL (19.7 µmol/L) (normal, 160-340 mg/dL [4.7-10.0 µmol/L). These coagulation abnormalities were interpreted as being consistent with vitamin K deficiency or warfarin effect. However, the patient was not taking warfarin, and the vitamin K level was not determined. Prothrombin and factor V Leiden mutations were not detected. Findings were negative for antineutrophilic cytoplasmic antibodies (cANCA and pANCA), cryoglobulins, and cryofibrinogens.

THERAPEUTIC CHALLENGE

The patient presented with signs and symptoms consistent with calciphylaxis. The mortality rate varies with the distribution of involvement, with reported mortality rates ranging from 23% (distal) to 63% (proximal).1 Treatments reported beneficial for calciphylaxis include parathyroidectomy,13 low-molecular-weight heparin,4 wound debridement,2 hyperbaric oxygen,5,6 intensive wound care, and increased frequency of hemodialysis.7

In our patient, wound debridement and intensive local wound care had not led to improvement. Parathyroidectomy was not performed in view of its uncertain benefit because of the patient's minimally increased level of parathyroid hormone. Hyperbaric oxygen treatment was not available.

SOLUTION

Because vascular occlusion may be a primary mechanism leading to the cutaneous necrosis associated with calciphylaxis,8,9 we elected to institute thrombolytic therapy in our patient. Low-dose tissue plasminogen activator (tPA; alteplase) was administered according to the protocol used for other cutaneous vaso-occlusive disorders (Table 1).10,11 The patient was hospitalized and given a daily 10-mg dose of alteplase intravenously for 14 days, followed by warfarin anticoagulation maintenance therapy. Also, the dialysis was intensified; the patient received hemodialysis daily for 1 week until the serum level of phosphorus normalized; then, he was maintained on hemodialysis 3 times weekly. Bedside debridement of the ulcers was performed daily as tolerated.

Low-Dose Tissue Plasminogen Activator Protocol*
Low-Dose Tissue Plasminogen Activator Protocol*

After completion of the tPA protocol (2 weeks after hospitalization), the ulcerated areas were markedly improved and less painful (Figure 1B). After discharge, the patient continued to receive wound care with daily whirlpool and periodic debridement. In addition, beginning at 6 weeks after discharge, skin grafts with the skin substitute Apligraf (Novartis, Basel, Switzerland) were placed on the larger areas of ulceration, with 4 total dressings applied over a 2-month period. Six months after tPA treatment, the patient had superficial erosions on the right and left anterior tibias, but otherwise the ulcers had healed completely (Figure 1C).

COMMENT

Thrombolytic therapy was considered a reasonable treatment choice for our patient for the following reasons: (1) there was clinical and laboratory evidence of a hypercoagulable state (history of multiple deep venous thromboses, low protein C level, and low antithrombin III level), and a hypercoagulable state has been reported in several patients with calciphylaxis1216; (2) fibrin thrombi were observed on histopathologic examination; (3) anticoagulation therapy has been reported effective in patients with calciphylaxis4; (4) we postulated that calciphylaxis may be considered an occlusive vasculopathy (with clinical resemblance to warfarin-induced skin necrosis or type I cryoglobulinemia); and (5) we have found that low-dose tPA is effective in the treatment of other coagulopathic disorders of the cutaneous microvasculature (eg, livedoid vasculopathy10,11 and antiphospholipid antibody syndrome). To our knowledge, therapies directed at clot lysis have not been used for calciphylaxis.

Tissue plasminogen activator has found wide use in reducing morbidity and mortality in thrombosis-associated myocardial infarction, pulmonary embolism, and stroke.17 We postulated that a mechanism similar to that occurring in atherosclerotic disease may mediate the pathogenesis of calciphylaxis, wherein progressive, calcific narrowing of the cutaneous microvasculature ultimately leads to thrombotic vascular occlusion and downstream tissue necrosis.

The explanation for the coagulopathy in our patient is unclear, but it may be secondary to poor nutrition, end-stage renal disease, or long-term hemodialysis or it may be multifactorial. Vitamin K deficiency has been observed in patients with chronic renal failure,18 in patients receiving long-term hemodialysis,19 and in patients with calciphylaxis.20,21

Thrombolytic therapy appeared to be effective in our patient. Paradoxically, oral anticoagulation with warfarin has been reported to be a risk factor for the development of calciphylaxis.4,22 This risk may be secondary to low vitamin K–dependent anticoagulation factors (eg, protein C and protein S). However, vitamin K replacement was associated with progression of calciphylaxis and mortality in a patient with calciphylaxis and a documented low serum level of vitamin K.20

Most treatments of calciphylaxis have centered on restoring calcium and phosphate homeostasis. Theoretically, this may prevent further calcification of the cutaneous microvasculature, which is thought to precede thrombus formation.23 These therapies have included parathyroidectomy (surgical and ethanol ablation), alteration of ionic concentrations in the dialysate, and oral phosphate binders.

Good wound care was likely a vital therapeutic component leading to healing in our patient. Regardless of the cause of cutaneous ulceration, debridement of necrotic tissue by surgery, whirlpool, or wet dressings is often necessary to allow for wound reepithelialization. Moreover, restoration of a normal phosphorus level after intensive dialysis may have contributed to our patient's improvement.

Because tPA therapy carries the risk of potential hemorrhage-associated morbidity and mortality, patients must be evaluated carefully for hemorrhagic tendencies before therapy is instituted. Furthermore, the tPA protocol is performed on an inpatient basis to monitor for hemorrhagic complications. Contraindications to tPA therapy may include a history of stroke, intracranial neoplasm, aneurysm, arteriovenous malformation, recent extracranial or spinal surgical procedure or trauma, active internal bleeding, bleeding diathesis, malignancy, or uncontrolled severe hypertension.

In summary, a hypercoagulable state may be the principal factor for the development of vascular occlusion and cutaneous necrosis in calciphylaxis. We suggest that a detailed coagulation screen be performed for patients with calciphylaxis. Low-dose tPA or other thrombolytic agents may prove effective in treating calciphylaxis. However, our findings are limited to this report and need to be confirmed with further studies.

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Clinicians, local and regional societies, residents, and fellows are invited to submit cases of challenges in management and therapeutics to this section. Cases should follow the established pattern. Submit 4 double-spaced copies of the manuscript with right margins nonjustified and 4 sets of the illustrations. Photomicrographs and illustrations must be clear and submitted as positive color transparencies (35-mm slides) or black-and-white prints. Do not submit color prints unless accompanied by original transparencies. Material should be accompanied by the required copyright transfer statement, as noted in "Instructions for Authors." Material for this section should be submitted to George J. Hruza, MD, Laser and Dermatologic Surgery Center Inc, 14377 Woodlake Dr, Suite 111, St Louis, MO 63017.

Corresponence: Roger H. Weenig, MD, Department of Dermatology, Mayo Clinic, Rochester, MN 55905 (weenig.roger@mayo.edu).

Accepted for publication October 14, 2003.

The authors have no relevant financial interest in this article.

References
1.
Hafner  JKeusch  GWahl  CBurg  G Calciphylaxis: a syndrome of skin necrosis and acral gangrene in chronic renal failure Vasa. 1998;27137- 143
PubMed
2.
Kang  ASMcCarthy  JTRowland  CFarley  DRvan Heerden  JA Is calciphylaxis best treated surgically or medically? Surgery. 2000;128967- 971
PubMedArticle
3.
Girotto  JAHarmon  JWRatner  LENicol  TLWong  LChen  H Parathyroidectomy promotes wound healing and prolongs survival in patients with calciphylaxis from secondary hyperparathyroidism Surgery. 2001;130645- 650
PubMedArticle
4.
Coates  TKirkland  GSDymock  RB  et al.  Cutaneous necrosis from calcific uremic arteriolopathy Am J Kidney Dis. 1998;32384- 391
PubMedArticle
5.
Podymow  TWherrett  CBurns  KD Hyperbaric oxygen in the treatment of calciphylaxis: a case series Nephrol Dial Transplant. 2001;162176- 2180
PubMedArticle
6.
Dean  SMWerman  H Calciphylaxis: a favorable outcome with hyperbaric oxygen Vasc Med. 1998;3115- 120
PubMedArticle
7.
Russell  RBrookshire  MAZekonis  MMoe  SM Distal calcific uremic arteriolopathy in a hemodialysis patient responds to lowering of Ca x P product and aggressive wound care Clin Nephrol. 2002;58238- 243
PubMedArticle
8.
Janigan  DTHirsch  DJKlassen  GAMacDonald  AS Calcified subcutaneous arterioles with infarcts of the subcutis and skin ("calciphylaxis") in chronic renal failure Am J Kidney Dis. 2000;35588- 597
PubMedArticle
9.
Dahl  PRWinkelmann  RKConnolly  SM The vascular calcification-cutaneous necrosis syndrome J Am Acad Dermatol. 1995;3353- 58
PubMedArticle
10.
Klein  KLPittelkow  MR Tissue plasminogen activator for treatment of livedoid vasculitis Mayo Clin Proc. 1992;67923- 933
PubMedArticle
11.
Srinivasan  SKPittelkow  MRCooper Jr  LT Recombinant tissue plasminogen activator for the treatment of cutaneous infarctions in antiphospholipid antibody syndrome: a case report Angiology. 2001;52635- 639
PubMedArticle
12.
Korkmaz  CDundar  EZubaroglu  I Calciphylaxis in a patient with rheumatoid arthritis without renal failure and hyperparathyroidism: the possible role of long-term steroid use and protein S deficiency Clin Rheumatol. 2002;2166- 69
PubMedArticle
13.
Goldsmith  DJ Calciphylaxis, thrombotic diathesis and defects in coagulation regulation [letter] Nephrol Dial Transplant. 1997;121082- 1083
PubMedArticle
14.
Perez-Mijares  RGuzman-Zamudio  JLPayan-Lopez  JRodriguez-Fernandez  AGomez-Fernandez  PAlmaraz-Jimenez  M Calciphylaxis in a haemodialysis patient: functional protein S deficiency? Nephrol Dial Transplant. 1996;111856- 1859
PubMedArticle
15.
Rostaing  Lel Feki  SDelisle  MB  et al.  Calciphylaxis in a chronic hemodialysis patient with protein S deficiency Am J Nephrol. 1995;15524- 527
PubMedArticle
16.
Mehta  RLScott  GSloand  JAFrancis  CW Skin necrosis associated with acquired protein C deficiency in patients with renal failure and calciphylaxis Am J Med. 1990;88252- 257
PubMedArticle
17.
Toombs  CF New directions in thrombolytic therapy Curr Opin Pharmacol. 2001;1164- 168
PubMedArticle
18.
Malyszko  JWolczynski  SSkrzydlewska  EMalyszko  JSMysliwiec  M Vitamin K status in relation to bone metabolism in patients with renal failure Am J Nephrol. 2002;22504- 508
PubMedArticle
19.
Kohlmeier  MSaupe  JShearer  MJSchaefer  KAsmus  G Bone health of adult hemodialysis patients is related to vitamin K status Kidney Int. 1997;511218- 1221
PubMedArticle
20.
Riegert-Johnson  DLKaur  JSPfeifer  EA Calciphylaxis associated with cholangiocarcinoma treated with low-molecular-weight heparin and vitamin K Mayo Clin Proc. 2001;76749- 752
PubMedArticle
21.
Soundararajan  RLeehey  DJYu  AWIng  TSMiller  JB Skin necrosis and protein C deficiency associated with vitamin K depletion in a patient with renal failure Am J Med. 1992;93467- 470
PubMedArticle
22.
Alain  JPoulin  YPCloutier  RAGagne  EBaril  J Calciphylaxis: seven new cases J Cutan Med Surg. 2000;4213- 218
PubMed
23.
Au  SCrawford  RI Three-dimensional analysis of a calciphylaxis plaque: clues to pathogenesis J Am Acad Dermatol. 2002;4753- 57
PubMedArticle
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