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Article
October 1924

STUDIES IN THE DIASTATIC ACTIVITY OF THE BLOOD, WITH A CONSIDERATION OF ITS VALUE IN CLINICAL DIAGNOSIS

Author Affiliations

PORTLAND, ORE.

From the Medical Clinic of the Peter Bent Brigham Hospital, Boston.

Arch Intern Med (Chic). 1924;34(4):542-552. doi:10.1001/archinte.1924.00120040128012
Abstract

In 1917, Myers and Killian1 published a simple, delicate and accurate method for estimating the diastatic activity of the blood. Briefly, the method consists in allowing a known quantity of blood (2 c.c.) to act on a known quantity of pure soluble starch (1 c.c. of a 1 per cent. solution = 10 mg.) for a definite length of time (fifteen minutes) in a water bath at 40 C. The glucose thus produced by the blood diastase is then determined by the Myers and Bailey method.2 A control is run in a second tube to determine the original glucose contained in the 2 c.c. of blood, which is deducted from the total glucose found in the first tube. The results are recorded in terms of the percentage of the starch (10 mg.) transformed to reducing sugar (calculated as glucose) by the 2 c.c. of blood employed. Thus, by a "diastatic

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