That iron is a constant and important constituent of the hemoglobin of mammals has been recognized for many years, and in consequence it has played an important rôle in the treatment for anemias. Therefore, it is somewhat surprising to find so little evidence in the literature of studies of the blood iron. Although a few determinations of the iron content of the blood under varying conditions have been made, there has been little effort to utilize these figures as a means of distinguishing between the various types of anemia or to follow the changes that may occur in the hemoglobin during the course of treatment for anemia by means of iron or other substances.
The observations recorded in this paper were made particularly in order to determine another means of differentiating between the types of anemia and also to establish a satisfactory method of following the changes that may occur
MURPHY WP, LYNCH R, HOWARD IM. THE VALUE OF DETERMINATIONS OF THE IRON CONTENT OF WHOLE BLOOD. Arch Intern Med (Chic). 1931;47(6):883-892. doi:10.1001/archinte.1931.00140240056005