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In this thesis for the doctorate of medicine at the University of Copenhagen the author reviews a difficult subject, namely, that of blood lipids and fat tolerance. The thesis is published in English; the translation is passable.
The matter of blood lipids is confusing because of the well established fact that all the fats and lipoids of the blood are difficult to extract by means of the ordinary solvents of fat. Thus, different methods yield more or less of the various fractions, phospholipoids, cholesterol esters, free cholesterol and neutral fat. Nissen, in his investigation, limited his attention to the primary ethereal extract of dried blood. This, he says, contains all the neutral fat of the blood, together with the main part of the free cholesterol, little or no phosphorus to indicate the presence of any phospholipoid and no cholesterol esters. The micromethod of extraction of 0.1 cc. of dried blood
Studies on Alimentary Lipaemia in Man.. Arch Intern Med (Chic). 1935;55(5):871-872. doi:10.1001/archinte.1935.00160230164012