Photomicrography has been used to good advantage for a number of years to record for future reference or for purposes of demonstration the microscopic appearance of blood cells or tissue sections. That it might be used to demonstrate the actual shape of the erythrocyte and the location of granules in the leukocyte seemed possible, particularly after the introduction of the profile or three dimensional method of printing.
The problem which offered the most important possibility was that of demonstrating the spherocytes in the blood of a person suspected of having familial hemolytic anemia. However, in order to do this, it seemed desirable to visualize for comparison the erythrocytes of the blood of normal persons and those with other varieties of anemia.
In figure 1 is shown the photomicrograph of erythrocytes from a normal person as printed in the usual manner for comparison with the same erythrocytes recorded by the profile
MURPHY WP, GREEN ET. PROFILE PRINTING IN THE PHOTOMICROGRAPHY OF BLOOD CELLS. Arch Intern Med (Chic). 1943;71(6):814-826. doi:10.1001/archinte.1943.00210060075005