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The thesis of the author is that all cellular granules, colloidal particles, macromolecules, elementary bodies, and cellular debris are "microkaryocytes," which are defined as cells with a diameter of 0.1 to 0.2 micron, containing a distinct nucleus, and having a nuclear-cytoplasmic ratio of 1:2 or 1:3. The several hundred photomicrographs of dried cells and secretions stained with iodine or silver nitrate, purporting to show the cellular nature of the "microkaryocytes," were all taken with the oil-immersion lens of a standard microscope, but were subsequently enlarged to final magnifications of 4,000 to 20,000. The severe distortion of the normal cell structure by the drying and staining procedures used is evident from the sorry appearance of the few erythrocytes and leukocytes that can still be identified on the photographs. The impossibility of resolving a cell of 0.2 micron diameter into "nucleus and cytoplasm" with the light microscope needs no comment. The author
The Microkaryocytes, The Fourth Corpuscles and Their Functions.. AMA Arch Intern Med. 1951;88(2):268. doi:10.1001/archinte.1951.03810080136022