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Hunter's monograph gives "the principles underlying the quantitation of pigment mixtures in general, and the mixture of certain hemoglobin derivatives in particular...." The first 50 pages of the book are devoted to the theory of and the mathematical computations involved in the spectrophotometric analysis of two- and three-component systems. There are instructions for the preparation of conversion charts and nomograms. In the second 50 pages there are procedures for the spectrophotometric determination of such hemoglobin pigments as oxyhemoglobin, carbon monoxide hemoglobin, and neutral hemoglobin. Spectrophotometric methods for the determination of oxygen saturation and erythrocyte volume are also given. Unfortunately, no data are presented to show the reliability or advantages of these techniques when compared with the procedures currently being used in many clinical laboratories. The last section of the book presents the charts and nomograms secured by the author for the hemoglobin derivatives mentioned in the text.
For those laboratories
The Quantitation of Mixtures of Hemoglobin Derivatives by Photoelectric Spectrophotometry.. AMA Arch Intern Med. 1952;89(1):165-166. doi:10.1001/archinte.1952.00240010175019