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October 1969

Radioimmunoassay of Parathyroid Hormone

Author Affiliations

Bethesda, Md; Boston

From the Section on Mineral Metabolism, Metabolic Diseases Branch, National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Be-; thesda, Md; and the Endocrine Unit, Massachusetts General Hospital, Boston.

Arch Intern Med. 1969;124(4):413-416. doi:10.1001/archinte.1969.00300200025004

The technic of radioimmunoassay developed by Berson and Yalow 1 is based on the competitive inhibition of binding of radioactively labeled hormone to specific antibody. There must be available pure hormone antigen for labeling with radioisotope, usually iodine 131 or iodine 125. The ratio of antibody-bound hormone labeled with 131I (B) to free hormone labeled with 131I (F) is a function of hormone concentration. By measuring the ratio B/F after incubation with dilute mixtures of antibody and labeled hormone, a sensitive immunoassay can be obtained which can be applied to detection of hormone in peripheral plasma. A standard curve for the assay of parathyroid hormone is illustrated in Fig 1. The graphical analysis shown provides more than quantitation alone; by analyzing the shape of the curves one can derive important qualitative information regarding the nature of an unknown hormone sample. For example, a hormonal preparation immunological identical to

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