The lipids from normal beagle dog lungs, pulmonary surfactant, and exfoliated lining cells were quantitated by established procedures. The surfactant and cells were obtained by endobronchial saline lavage. Phospholipids accounted for more than 70% of the surfactant and cell lipids: phosphatidyl choline was the predominant phosphatide. Phosphatidyl glycerol is present in beagle dog pulmonary surfactant (10% of phospholipid-P), exfoliated lining cells (7% of phospholipid-P) and lung tissue (3% of phospholipid-P). Cholesterol and its esters accounted for more than 60% of the neutral lipid fractions. Alkyl and alk-1-enyl glycerol ethers were quantitated in the neutral lipids and phospholipids from all samples.
Pfleger RC, Thomas HG. Beagle Dog Pulmonary Surfactant LipidsLipid Composition of Pulmonary Tissue, Exfoliated Lining Cells, and Surfactant. Arch Intern Med. 1971;127(5):863–872. doi:10.1001/archinte.1971.00310170071010