January 1975

In Vitro and in Vivo Alterations of Long-Term Murine Plasmacytoma Cultures

Author Affiliations

From the Department of Pathology, Dartmouth Medical School, Hanover, NH.

Arch Intern Med. 1975;135(1):114-118. doi:10.1001/archinte.1975.00330010116015

Mouse myeloma cell line (SLU-5) that has undergone lengthy propagation in vitro and become nononcogenic was compared with an earlier oncogenic passage of cells. Immunogenicity of cells cultured for various periods of time was compared with that of the original tumor (MOPC-21). The nononcogenic cells were most immunogenic. Cells that were nononcogenic in normal mice produced tumors in nonlethally irradiated mice. Clones isolated from oncogenic and nononcogenic populations varied widely with respect to ability to produce tumors in mice and to specific globulin production.