May 1985

Tissue Culture Isolation of Toxoplasma From Blood of a Patient With AIDS

Author Affiliations

From the Department of Immunology and Infectious Diseases, Research Institute, Palo Alto (Calif) Medical Foundation, and Division of Infectious Diseases, Department of Medicine, Stanford (Calif) University Medical Center.

Arch Intern Med. 1985;145(5):925-926. doi:10.1001/archinte.1985.00360050195035

• A man with acquired immunodeficiency syndrome and multiple opportunistic infections developed central nervous system signs, and results of computed tomography suggested brain abscess. Histologic study and culture of material obtained from the brain biopsy specimen revealed Aspergillus. The clot from a peripheral venous blood sample obtained at the time of biopsy was processed and cultured on a monolayer of L cells. Five days later, Toxoplasma tachyzoites were identified in the cell culture. We bring this case to the attention of the medical community to point out that inoculation of tissue cell cultures as for viral isolation may aid in the diagnosis of Toxoplasma infection in these and other immunocompromised patients.

(Arch Intern Med 1985;145:925-926)